VIP Inhibits P. gingivalis LPS-induced IL-18 and IL-18BPa in Monocytes

IL-18 is a pro-inflammatory cytokine that is important in the regulation of T-cells and is elevated in inflammatory disorders such as periodontal disease. Vasoactive intestinal peptide (VIP) modulates immune responses to the periodontal pathogen Porphyromonas gingivalis (Pg). Our objective was to in...

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Published in:Journal of dental research 2007-09, Vol.86 (9), p.883-887
Main Authors: Foster, N., Andreadou, K., Jamieson, L., Preshaw, P.M., Taylor, J.J.
Format: Article
Language:English
Subjects:
Analysis of Variance
Cell Line
Dentistry
Humans
Inflammation Mediators - pharmacology
Inflammation Mediators - physiology
Intercellular Signaling Peptides and Proteins - biosynthesis
Interleukin-18 - antagonists & inhibitors
Interleukin-18 - biosynthesis
Lipopolysaccharides - pharmacology
Monocytes - drug effects
Monocytes - immunology
Monocytes - metabolism
Porphyromonas gingivalis - chemistry
Vasoactive Intestinal Peptide - pharmacology
Vasoactive Intestinal Peptide - physiology
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cited_by cdi_FETCH-LOGICAL-c463t-71f3da7fa4fd42cd9d9c00c5b6cd2fb83f340858cbd8faef5aa5824b26c689573
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container_issue 9
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container_title Journal of dental research
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creator Foster, N.
Andreadou, K.
Jamieson, L.
Preshaw, P.M.
Taylor, J.J.
description IL-18 is a pro-inflammatory cytokine that is important in the regulation of T-cells and is elevated in inflammatory disorders such as periodontal disease. Vasoactive intestinal peptide (VIP) modulates immune responses to the periodontal pathogen Porphyromonas gingivalis (Pg). Our objective was to investigate the effect of Pg LPS on IL-18 and its natural inhibitor, IL-18 binding protein (IL-18BPa), in human monocytes, and the effect of VIP on this system. We demonstrated that Pg LPS induced both IL-18 and IL-18BPa secretion in cultures of the human monocytic cell line THP-1, as measured by specific ELISA. The addition of antibodies to IL-18BPa to the stimulated THP-1 cultures resulted in increased levels of free IL-18, indicating a specific interaction between IL18 and IL-18BPa in this system. VIP (10−8M) inhibited both IL-18 and IL-18Bpa secretion by stimulated monocytes. We conclude that IL-18 and IL-18BPa secretion by monocytes is part of the immune response to Pg, and that VIP can inhibit this process.
doi_str_mv 10.1177/154405910708600915
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Vasoactive intestinal peptide (VIP) modulates immune responses to the periodontal pathogen Porphyromonas gingivalis (Pg). Our objective was to investigate the effect of Pg LPS on IL-18 and its natural inhibitor, IL-18 binding protein (IL-18BPa), in human monocytes, and the effect of VIP on this system. We demonstrated that Pg LPS induced both IL-18 and IL-18BPa secretion in cultures of the human monocytic cell line THP-1, as measured by specific ELISA. The addition of antibodies to IL-18BPa to the stimulated THP-1 cultures resulted in increased levels of free IL-18, indicating a specific interaction between IL18 and IL-18BPa in this system. VIP (10−8M) inhibited both IL-18 and IL-18Bpa secretion by stimulated monocytes. 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ispartof Journal of dental research, 2007-09, Vol.86 (9), p.883-887
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source Sage Journals Online
subjects Analysis of Variance
Cell Line
Dentistry
Humans
Inflammation Mediators - pharmacology
Inflammation Mediators - physiology
Intercellular Signaling Peptides and Proteins - biosynthesis
Interleukin-18 - antagonists & inhibitors
Interleukin-18 - biosynthesis
Lipopolysaccharides - pharmacology
Monocytes - drug effects
Monocytes - immunology
Monocytes - metabolism
Porphyromonas gingivalis - chemistry
Vasoactive Intestinal Peptide - pharmacology
Vasoactive Intestinal Peptide - physiology
title VIP Inhibits P. gingivalis LPS-induced IL-18 and IL-18BPa in Monocytes
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