Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells

The aim of this study was to evaluate the survival responses of four strains of Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25 °C). V. alginolyticus maintained its culturability for at least...

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Bibliographic Details
Published in:Marine environmental research 2007-10, Vol.64 (4), p.469-478
Main Authors: Ben Kahla-Nakbi, Amel, Besbes, Amine, Chaieb, Kamel, Rouabhia, Mahmoud, Bakhrouf, Amina
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Animal and plant ecology
Animal, plant and microbial ecology
Animals
ARDRA
Bass - microbiology
Biological and medical sciences
Dicentrarchus labrax
DNA, Ribosomal - classification
Fundamental and applied biological sciences. Psychology
Marine
Sea Bream - microbiology
Sea water ecosystems
Seawater
Seawater - microbiology
Sparus aurata
Survival
Synecology
Temperature
VBNC
Vibrio alginolyticus
Vibrio alginolyticus - classification
Vibrio alginolyticus - pathogenicity
Vibrio alginolyticus - physiology
Virulence
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Summary:The aim of this study was to evaluate the survival responses of four strains of Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25 °C). V. alginolyticus maintained its culturability for at least nine months. Long-term-starved cells showed an absence or a decrease in their enzymatic activities. Resuscitation assays of viable but non-culturable (VBNC) cells were conducted and the recovery of these cells was achieved after the addition of nutrients. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that the same strain of V. alginolyticus persisted in all microcosms during a long period of time. Starved cells maintained their infectivity for gilt head sea bream ( Sparus aurata) and sea bass ( Dicentrachus labrax) as determined by intraperitoneal challenges.
ISSN:0141-1136
1879-0291
DOI:10.1016/j.marenvres.2007.04.002