Loading…
Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells
The aim of this study was to evaluate the survival responses of four strains of Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25 °C). V. alginolyticus maintained its culturability for at least...
Saved in:
| Published in: | Marine environmental research 2007-10, Vol.64 (4), p.469-478 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733 |
| container_end_page | 478 |
| container_issue | 4 |
| container_start_page | 469 |
| container_title | Marine environmental research |
| container_volume | 64 |
| creator | Ben Kahla-Nakbi, Amel Besbes, Amine Chaieb, Kamel Rouabhia, Mahmoud Bakhrouf, Amina |
| description | The aim of this study was to evaluate the survival responses of four strains of
Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25
°C).
V. alginolyticus maintained its culturability for at least nine months. Long-term-starved cells showed an absence or a decrease in their enzymatic activities. Resuscitation assays of viable but non-culturable (VBNC) cells were conducted and the recovery of these cells was achieved after the addition of nutrients. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that the same strain of
V. alginolyticus persisted in all microcosms during a long period of time. Starved cells maintained their infectivity for gilt head sea bream (
Sparus aurata) and sea bass (
Dicentrachus labrax) as determined by intraperitoneal challenges. |
| doi_str_mv | 10.1016/j.marenvres.2007.04.002 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68202863</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141113607000578</els_id><sourcerecordid>68202863</sourcerecordid><originalsourceid>FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhi0EokvhL0AucEuYcRw7OVZV-ZAqceDjhGTNOhPkVdYpthPUf0-iXdFjOY0sPa_n1TxCvEGoEFC_P1RHihyWyKmSAKYCVQHIJ2KHrelKkB0-FTtAhSVirS_Ei5QOANAYbJ6LCzSNVMrUO_Hz6xwXv9BYTEPxw--jnwoaf_kwjffZuzkVPhSJ6Q9ljgWFvoicOWQ_hS2x-DiPHBxvD59TkTLFhfvC8Timl-LZQGPiV-d5Kb5_uPl2_am8_fLx8_XVbekao3NZY6fVXulB1a51HaHsle41Dk2HDE621JOivTFtq6lxHTYoDRFSP6BDU9eX4t3p37s4_Z45ZXv0aWtAgac5Wd1KkK1-HJSAEg12_wFCrU2tVtCcQBenlCIP9i761c29RbCbKnuw_1RtMWNB2VXVmnx9XjHvj9w_5M5uVuDtGaDkaBwiBefTA9eBAgNbhasTx-uJF8_RJuc3J72P7LLtJ_9omb_jfLam</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20036734</pqid></control><display><type>article</type><title>Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells</title><source>ScienceDirect Freedom Collection</source><creator>Ben Kahla-Nakbi, Amel ; Besbes, Amine ; Chaieb, Kamel ; Rouabhia, Mahmoud ; Bakhrouf, Amina</creator><creatorcontrib>Ben Kahla-Nakbi, Amel ; Besbes, Amine ; Chaieb, Kamel ; Rouabhia, Mahmoud ; Bakhrouf, Amina</creatorcontrib><description>The aim of this study was to evaluate the survival responses of four strains of
Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25
°C).
V. alginolyticus maintained its culturability for at least nine months. Long-term-starved cells showed an absence or a decrease in their enzymatic activities. Resuscitation assays of viable but non-culturable (VBNC) cells were conducted and the recovery of these cells was achieved after the addition of nutrients. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that the same strain of
V. alginolyticus persisted in all microcosms during a long period of time. Starved cells maintained their infectivity for gilt head sea bream (
Sparus aurata) and sea bass (
Dicentrachus labrax) as determined by intraperitoneal challenges.</description><identifier>ISSN: 0141-1136</identifier><identifier>EISSN: 1879-0291</identifier><identifier>DOI: 10.1016/j.marenvres.2007.04.002</identifier><identifier>PMID: 17524473</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adaptation, Physiological ; Animal and plant ecology ; Animal, plant and microbial ecology ; Animals ; ARDRA ; Bass - microbiology ; Biological and medical sciences ; Dicentrarchus labrax ; DNA, Ribosomal - classification ; Fundamental and applied biological sciences. Psychology ; Marine ; Sea Bream - microbiology ; Sea water ecosystems ; Seawater ; Seawater - microbiology ; Sparus aurata ; Survival ; Synecology ; Temperature ; VBNC ; Vibrio alginolyticus ; Vibrio alginolyticus - classification ; Vibrio alginolyticus - pathogenicity ; Vibrio alginolyticus - physiology ; Virulence</subject><ispartof>Marine environmental research, 2007-10, Vol.64 (4), p.469-478</ispartof><rights>2007 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733</citedby><cites>FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19040704$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17524473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ben Kahla-Nakbi, Amel</creatorcontrib><creatorcontrib>Besbes, Amine</creatorcontrib><creatorcontrib>Chaieb, Kamel</creatorcontrib><creatorcontrib>Rouabhia, Mahmoud</creatorcontrib><creatorcontrib>Bakhrouf, Amina</creatorcontrib><title>Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells</title><title>Marine environmental research</title><addtitle>Mar Environ Res</addtitle><description>The aim of this study was to evaluate the survival responses of four strains of
Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25
°C).
V. alginolyticus maintained its culturability for at least nine months. Long-term-starved cells showed an absence or a decrease in their enzymatic activities. Resuscitation assays of viable but non-culturable (VBNC) cells were conducted and the recovery of these cells was achieved after the addition of nutrients. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that the same strain of
V. alginolyticus persisted in all microcosms during a long period of time. Starved cells maintained their infectivity for gilt head sea bream (
Sparus aurata) and sea bass (
Dicentrachus labrax) as determined by intraperitoneal challenges.</description><subject>Adaptation, Physiological</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>ARDRA</subject><subject>Bass - microbiology</subject><subject>Biological and medical sciences</subject><subject>Dicentrarchus labrax</subject><subject>DNA, Ribosomal - classification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Marine</subject><subject>Sea Bream - microbiology</subject><subject>Sea water ecosystems</subject><subject>Seawater</subject><subject>Seawater - microbiology</subject><subject>Sparus aurata</subject><subject>Survival</subject><subject>Synecology</subject><subject>Temperature</subject><subject>VBNC</subject><subject>Vibrio alginolyticus</subject><subject>Vibrio alginolyticus - classification</subject><subject>Vibrio alginolyticus - pathogenicity</subject><subject>Vibrio alginolyticus - physiology</subject><subject>Virulence</subject><issn>0141-1136</issn><issn>1879-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhi0EokvhL0AucEuYcRw7OVZV-ZAqceDjhGTNOhPkVdYpthPUf0-iXdFjOY0sPa_n1TxCvEGoEFC_P1RHihyWyKmSAKYCVQHIJ2KHrelKkB0-FTtAhSVirS_Ei5QOANAYbJ6LCzSNVMrUO_Hz6xwXv9BYTEPxw--jnwoaf_kwjffZuzkVPhSJ6Q9ljgWFvoicOWQ_hS2x-DiPHBxvD59TkTLFhfvC8Timl-LZQGPiV-d5Kb5_uPl2_am8_fLx8_XVbekao3NZY6fVXulB1a51HaHsle41Dk2HDE621JOivTFtq6lxHTYoDRFSP6BDU9eX4t3p37s4_Z45ZXv0aWtAgac5Wd1KkK1-HJSAEg12_wFCrU2tVtCcQBenlCIP9i761c29RbCbKnuw_1RtMWNB2VXVmnx9XjHvj9w_5M5uVuDtGaDkaBwiBefTA9eBAgNbhasTx-uJF8_RJuc3J72P7LLtJ_9omb_jfLam</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Ben Kahla-Nakbi, Amel</creator><creator>Besbes, Amine</creator><creator>Chaieb, Kamel</creator><creator>Rouabhia, Mahmoud</creator><creator>Bakhrouf, Amina</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>H95</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells</title><author>Ben Kahla-Nakbi, Amel ; Besbes, Amine ; Chaieb, Kamel ; Rouabhia, Mahmoud ; Bakhrouf, Amina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adaptation, Physiological</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>ARDRA</topic><topic>Bass - microbiology</topic><topic>Biological and medical sciences</topic><topic>Dicentrarchus labrax</topic><topic>DNA, Ribosomal - classification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Marine</topic><topic>Sea Bream - microbiology</topic><topic>Sea water ecosystems</topic><topic>Seawater</topic><topic>Seawater - microbiology</topic><topic>Sparus aurata</topic><topic>Survival</topic><topic>Synecology</topic><topic>Temperature</topic><topic>VBNC</topic><topic>Vibrio alginolyticus</topic><topic>Vibrio alginolyticus - classification</topic><topic>Vibrio alginolyticus - pathogenicity</topic><topic>Vibrio alginolyticus - physiology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ben Kahla-Nakbi, Amel</creatorcontrib><creatorcontrib>Besbes, Amine</creatorcontrib><creatorcontrib>Chaieb, Kamel</creatorcontrib><creatorcontrib>Rouabhia, Mahmoud</creatorcontrib><creatorcontrib>Bakhrouf, Amina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>MEDLINE - Academic</collection><jtitle>Marine environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ben Kahla-Nakbi, Amel</au><au>Besbes, Amine</au><au>Chaieb, Kamel</au><au>Rouabhia, Mahmoud</au><au>Bakhrouf, Amina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells</atitle><jtitle>Marine environmental research</jtitle><addtitle>Mar Environ Res</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>64</volume><issue>4</issue><spage>469</spage><epage>478</epage><pages>469-478</pages><issn>0141-1136</issn><eissn>1879-0291</eissn><abstract>The aim of this study was to evaluate the survival responses of four strains of
Vibrio alginolyticus in seawater under starvation conditions. We used microcosms containing sterilised seawater and incubated at ambient temperature (22–25
°C).
V. alginolyticus maintained its culturability for at least nine months. Long-term-starved cells showed an absence or a decrease in their enzymatic activities. Resuscitation assays of viable but non-culturable (VBNC) cells were conducted and the recovery of these cells was achieved after the addition of nutrients. Amplified 16S ribosomal DNA (rDNA) restriction analysis (ARDRA) was used to confirm that the same strain of
V. alginolyticus persisted in all microcosms during a long period of time. Starved cells maintained their infectivity for gilt head sea bream (
Sparus aurata) and sea bass (
Dicentrachus labrax) as determined by intraperitoneal challenges.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>17524473</pmid><doi>10.1016/j.marenvres.2007.04.002</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-1136 |
| ispartof | Marine environmental research, 2007-10, Vol.64 (4), p.469-478 |
| issn | 0141-1136 1879-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68202863 |
| source | ScienceDirect Freedom Collection |
| subjects | Adaptation, Physiological Animal and plant ecology Animal, plant and microbial ecology Animals ARDRA Bass - microbiology Biological and medical sciences Dicentrarchus labrax DNA, Ribosomal - classification Fundamental and applied biological sciences. Psychology Marine Sea Bream - microbiology Sea water ecosystems Seawater Seawater - microbiology Sparus aurata Survival Synecology Temperature VBNC Vibrio alginolyticus Vibrio alginolyticus - classification Vibrio alginolyticus - pathogenicity Vibrio alginolyticus - physiology Virulence |
| title | Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T01%3A45%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Survival%20of%20Vibrio%20alginolyticus%20in%20seawater%20and%20retention%20of%20virulence%20of%20its%20starved%20cells&rft.jtitle=Marine%20environmental%20research&rft.au=Ben%20Kahla-Nakbi,%20Amel&rft.date=2007-10-01&rft.volume=64&rft.issue=4&rft.spage=469&rft.epage=478&rft.pages=469-478&rft.issn=0141-1136&rft.eissn=1879-0291&rft_id=info:doi/10.1016/j.marenvres.2007.04.002&rft_dat=%3Cproquest_cross%3E68202863%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c576t-31964b46f43c8c9a12d46d61f591e0c28ada4ab77886a5c915127aa1adf1c1733%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20036734&rft_id=info:pmid/17524473&rfr_iscdi=true |