A facile and effective synthesis of dinucleotide 5′-triphosphates

Successful synthetic procedure for the conversion of 5′-monophosphorylated 2′-deoxydinucleotides into their 5′-triphosphate derivatives in satisfactory to excellent yields. We report on the successful synthetic procedure for the conversion of 5′-monophosphorylated 2′-deoxydinucleotides into their 5′...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry 2007-10, Vol.15 (20), p.6549-6555
Main Authors: Abramova, Tatiana V., Vasileva, Svetlana V., Serpokrylova, Inna Yu, Kless, Hadar, Silnikov, Vladimir N.
Format: Article
Language:English
Subjects:
Chromatography, Ion Exchange
Dinucleoside Phosphates - chemical synthesis
Dinucleoside Phosphates - chemistry
Furans - chemistry
Kinetics
Magnetic Resonance Spectroscopy
Molecular Structure
Oligonucleotides
Stereoisomerism
Terminal phosphate activation
Triphosphates
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Summary:Successful synthetic procedure for the conversion of 5′-monophosphorylated 2′-deoxydinucleotides into their 5′-triphosphate derivatives in satisfactory to excellent yields. We report on the successful synthetic procedure for the conversion of 5′-monophosphorylated 2′-deoxydinucleotides into their 5′-triphosphate derivatives in satisfactory to excellent yields. The activation of the terminal phosphate group was achieved under the Mukaiyama conditions in the presence of a nucleophilic catalyst. The reaction conditions (solvent, counter ions, activation time and reagent excess) were optimized for all dinucleotides.
ISSN:0968-0896
1464-3391
DOI:10.1016/j.bmc.2007.07.002