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The 3.6 kb DNA fragment from the rat Col1a1 gene promoter drives the expression of genes in both osteoblast and osteoclast lineage cells

The type I collagen promoter has been used to develop transgenic constructs that are able to mark different stages of osteoblastic differentiation. The pOBCol3.6 promoter is active in early mesenchymal progenitors, including preosteoblasts and osteoblasts, while the pOBCol2.3 promoter is more restri...

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Published in:Bone (New York, N.Y.) N.Y.), 2006-12, Vol.39 (6), p.1302-1312
Main Authors: Boban, Ivana, Jacquin, Claire, Prior, Katie, Barisic-Dujmovic, Tatjana, Maye, Peter, Clark, Stephen H., Aguila, Hector L.
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container_title Bone (New York, N.Y.)
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creator Boban, Ivana
Jacquin, Claire
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Aguila, Hector L.
description The type I collagen promoter has been used to develop transgenic constructs that are able to mark different stages of osteoblastic differentiation. The pOBCol3.6 promoter is active in early mesenchymal progenitors, including preosteoblasts and osteoblasts, while the pOBCol2.3 promoter is more restricted, showing expression in mature osteoblasts and osteocytes. Transgenic mouse lines have been created that express various GFP reporters under the control of both promoters. These transgenic mice permit the tracking of osteoblastic lineage progression in vitro. They also represent a system to test lineage progression in vivo after the transplantation of progenitors. A parabiosis system was used in which pOBCol3.6GFP transgenic mice were surgically joined with mice bearing a Col2.3ΔTK transgene. The Col2.3ΔTK transgenic mouse bears a herpes thymidine kinase gene driven by the pOBCol2.3 promoter, and upon treatment with gancyclovir (GCV) displays extensive destruction of the bone lining cells. After a common circulation was established, parabiotic pairs were treated with GCV for 15 days. Histological analysis of their bones showed the clear presence of GFP positive cells in the Col2.3ΔTK parabionts, around trabecular bone and on the endosteal and periosteal surfaces. Stromal cell cultures from these Col2.3ΔTK parabionts did not display mineralized colonies coexpressing GFP. In contrast, scattered GFP positive clusters that contained large cells with morphology similar to osteoclast like cells (OCLs) were observed. These cells were also TRAP positive. They were readily detected in Col2.3ΔTK mice treated with GCV and transplanted with purified hematopoietic stem cells (HSCs) isolated from pOBCol3.6GFP mice. OCLs were also generated in vitro from osteoclast progenitor cells obtained from pOBCol3.6GFP mice that were defined by the B220− CD3− CD11b− c-fms+ phenotype. Molecular analysis showed that OCLs did not express type I collagen indicating that the Col3.6 promoter contains elements that are active during osteoclastogenesis and are not strictly related to collagen transcription. In summary, we demonstrate that pOBCol3.6 unexpectedly directs the expression of transgenes in the osteoclast lineage and this effect must be considered when utilizing this promoter to study of mesenchymal progenitor cells.
doi_str_mv 10.1016/j.bone.2006.06.025
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The pOBCol3.6 promoter is active in early mesenchymal progenitors, including preosteoblasts and osteoblasts, while the pOBCol2.3 promoter is more restricted, showing expression in mature osteoblasts and osteocytes. Transgenic mouse lines have been created that express various GFP reporters under the control of both promoters. These transgenic mice permit the tracking of osteoblastic lineage progression in vitro. They also represent a system to test lineage progression in vivo after the transplantation of progenitors. A parabiosis system was used in which pOBCol3.6GFP transgenic mice were surgically joined with mice bearing a Col2.3ΔTK transgene. The Col2.3ΔTK transgenic mouse bears a herpes thymidine kinase gene driven by the pOBCol2.3 promoter, and upon treatment with gancyclovir (GCV) displays extensive destruction of the bone lining cells. After a common circulation was established, parabiotic pairs were treated with GCV for 15 days. Histological analysis of their bones showed the clear presence of GFP positive cells in the Col2.3ΔTK parabionts, around trabecular bone and on the endosteal and periosteal surfaces. Stromal cell cultures from these Col2.3ΔTK parabionts did not display mineralized colonies coexpressing GFP. In contrast, scattered GFP positive clusters that contained large cells with morphology similar to osteoclast like cells (OCLs) were observed. These cells were also TRAP positive. They were readily detected in Col2.3ΔTK mice treated with GCV and transplanted with purified hematopoietic stem cells (HSCs) isolated from pOBCol3.6GFP mice. OCLs were also generated in vitro from osteoclast progenitor cells obtained from pOBCol3.6GFP mice that were defined by the B220− CD3− CD11b− c-fms+ phenotype. Molecular analysis showed that OCLs did not express type I collagen indicating that the Col3.6 promoter contains elements that are active during osteoclastogenesis and are not strictly related to collagen transcription. In summary, we demonstrate that pOBCol3.6 unexpectedly directs the expression of transgenes in the osteoclast lineage and this effect must be considered when utilizing this promoter to study of mesenchymal progenitor cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16938497</pmid><doi>10.1016/j.bone.2006.06.025</doi><tpages>11</tpages></addata></record>
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identifier ISSN: 8756-3282
ispartof Bone (New York, N.Y.), 2006-12, Vol.39 (6), p.1302-1312
issn 8756-3282
1873-2763
language eng
recordid cdi_proquest_miscellaneous_68206866
source Elsevier
subjects Animals
Base Sequence
Bone Marrow Transplantation
Collagen
Collagen Type I - genetics
DNA - genetics
Female
Ganciclovir - pharmacology
Gene Expression
Green fluorescent protein
Green Fluorescent Proteins - genetics
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Osteoblast progenitors
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteoclast
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - metabolism
Parabiosis
Promoter Regions, Genetic
Rats
Recombinant Proteins - genetics
Transplantation Chimera
title The 3.6 kb DNA fragment from the rat Col1a1 gene promoter drives the expression of genes in both osteoblast and osteoclast lineage cells
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