Further analysis of anti-human leukocyte mAbs with reactivity to equine leukocytes by two-colour flow cytometry and immunohistochemistry

We have reported on the reactivity of anti-human CD molecules with equine leukocytes by single-colour flow cytometry (this issue). The objectives of this additional study were to test for the reliability of the results obtained, and to obtain further information on the positive populations of lympho...

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Bibliographic Details
Published in:Veterinary Immunology and Immunopathology 2007-09, Vol.119 (1), p.92-99
Main Authors: Flaminio, M. Julia B.F., Ibrahim, Sherif, Lunn, D. Paul, Stark, Robert, Steinbach, Falko
Format: Article
Language:English
Subjects:
Animals
anti-human leukocyte monoclonal antibodies
Antibodies, Monoclonal - immunology
Antigens, CD - analysis
Antigens, CD - immunology
cell differentiation
cross reaction
Cross Reactions
Flow cytometry
Flow Cytometry - methods
Horse
horses
Horses - immunology
Humans
Immunohistochemistry
Immunology
Leukocyte differentiation
leukocytes
Leukocytes - immunology
monoclonal antibodies
sequence homology
two-color flow cytometry
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Summary:We have reported on the reactivity of anti-human CD molecules with equine leukocytes by single-colour flow cytometry (this issue). The objectives of this additional study were to test for the reliability of the results obtained, and to obtain further information on the positive populations of lymphocytes. Two-colour flow cytometry and immunohistochemistry were performed, using many of the positive mAbs and a few questionable ones from the first part of the study. All mAbs analysed by two-colour flow cytometry could be confirmed to their previous designation as “positive” or “questionable”. Most of the mAbs tested were effective in immunohistochemistry, supporting previous results. Examples of positive results will be presented and limitations of the study will be discussed briefly.
ISSN:0165-2427
1873-2534
1365-2567
DOI:10.1016/j.vetimm.2007.06.035