Phosphorylation of Irs1 at SER-522 Inhibits Insulin Signaling

Multisite phosphorylation of Irs1 on serine and threonine residues regulates insulin signaling that can contribute to insulin resistance. We identified by mass spectrometry the phosphorylation of Ser522 in rat Irs1 (S522Irs1). The functional effects of this phosphorylation site were investigated in...

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Bibliographic Details
Published in:Molecular endocrinology (Baltimore, Md.) Md.), 2007-09, Vol.21 (9), p.2294-2302
Main Authors: Giraud, Jodel, Haas, Michael, Feener, Edward P, Copps, Kyle D, Dong, Xiaocheng, Dunn, Sarah L, White, Morris F
Format: Article
Language:English
Subjects:
Animals
CHO Cells
Cricetinae
Cricetulus
Insulin - metabolism
Insulin Receptor Substrate Proteins
Phosphoproteins - genetics
Phosphoproteins - metabolism
Phosphorylation
Rabbits
Serine - genetics
Serine - metabolism
Signal Transduction - physiology
Threonine - genetics
Threonine - metabolism
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Summary:Multisite phosphorylation of Irs1 on serine and threonine residues regulates insulin signaling that can contribute to insulin resistance. We identified by mass spectrometry the phosphorylation of Ser522 in rat Irs1 (S522Irs1). The functional effects of this phosphorylation site were investigated in cultured cells using a sequence-specific phosphoserine antibody. Insulin stimulated the phosphorylation of S522Irs1 in L6 myoblasts and myotubes. S522Irs1 phosphorylation was inhibited by wortmannin, whereas PD98059, rapamycin, or glucose-starvation had no effect. Reducing Akt expression with small interfering RNA inhibited insulin-stimulated phosphorylation of S522Irs1, suggesting the involvement of the phosphatidylinositol 3-kinase→ Akt cascade. A S522Irs1→A522Irs1 substitution increased insulin-stimulated tyrosine phosphorylation of Irs1 and signaling, whereas a S522Irs1→E522Irs1 substitution reduced insulin-stimulated Irs1 tyrosine phosphorylation. Together, these results suggest the phosphatidylinositol 3-kinase→Akt cascade can inhibit insulin signaling through the phosphorylation of S522Irs1.
ISSN:0888-8809
1944-9917
DOI:10.1210/me.2007-0159