A simple in-vitro ‘wet-plate’ method for mass production of Phytophthora nicotianae zoospores and factors influencing zoospore production

A simple in-vitro ‘wet-plate’ method for mass-producing Phytophthora nicotianae zoospores at ≥ 1.0 × 10 6 zoospores/ml is described. Temperature critically affected zoospore production; 22 °C was optimum, while 36 °C was completely inhibitory. Zoospores being the most important propagule of P. nicot...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2007-09, Vol.70 (3), p.557-560
Main Authors: Ahonsi, Monday O., Banko, Thomas J., Hong, Chuanxue
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Crops, Agricultural
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Microbiology
Mycological methods and techniques used in mycology
Mycology
Other applications
Phytophthora
Phytophthora - growth & development
Phytophthora - microbiology
Phytophthora nicotianae
Recycled irrigation water
Spores, Fungal - growth & development
Temperature
Water Microbiology
Zoospore production
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple in-vitro ‘wet-plate’ method for mass-producing Phytophthora nicotianae zoospores at ≥ 1.0 × 10 6 zoospores/ml is described. Temperature critically affected zoospore production; 22 °C was optimum, while 36 °C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2007.06.012