Regional Morphogenesis in the Hypothalamus: A BMP-Tbx2 Pathway Coordinates Fate and Proliferation through Shh Downregulation

• Published in: Developmental cell 2006-12, Vol.11 (6), p.873-885
• Main Authors: Manning, Liz, Ohyama, Kyoji, Saeger, Bernhard, Hatano, Osamu, Wilson, Stuart A., Logan, Malcolm, Placzek, Marysia
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Bone Morphogenetic Proteins - genetics; Bone Morphogenetic Proteins - metabolism; Cell Cycle; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Cell Proliferation; Cells, Cultured; Chick Embryo; Chickens; Chlorocebus aethiops; COS Cells; DEVBIO; Down-Regulation; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Embryo, Nonmammalian; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Hedgehog Proteins - antagonists & inhibitors; Hedgehog Proteins - genetics; Hedgehog Proteins - physiology; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Hypothalamus - embryology; Hypothalamus - metabolism; Immunoenzyme Techniques; In Situ Hybridization; Mice; Molecular and cellular biology; Patched Receptors; Patched-1 Receptor; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; RNA, Small Interfering - pharmacology; Signal Transduction; Stem Cells - metabolism; T-Box Domain Proteins - antagonists & inhibitors; T-Box Domain Proteins - genetics; T-Box Domain Proteins - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Wnt Proteins - metabolism
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2006.09.021

A central challenge in embryonic development is to understand how growth and pattern are coordinated to direct emerging new territories during morphogenesis. Here, we report on a signaling cascade that links cell proliferation and fate, promoting formation of a distinct progenitor domain within the developing chick hypothalamus. We show that the downregulation of Shh in floor plate-like cells in the forebrain governs their progression to a distinctive, proliferating hypothalamic progenitor domain. Shh downregulation occurs via a local BMP-Tbx2 pathway, Tbx2 acting to repress Shh expression. We show in vivo and in vitro that forced maintenance of Shh in hypothalamic progenitors prevents their normal morphogenesis, leading to maintenance of the Shh receptor, ptc, and preventing progression to an Emx2 +-proliferative progenitor state. Our data identify a molecular pathway for the downregulation of Shh via a BMP-Tbx2 pathway and provide a mechanism for expansion of a discrete progenitor domain within the developing forebrain.