Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high-performance thin layer chromatography

A simple, precise and rapid high-performance thin-layer chromatographic method has been developed for the estimation of phyllanthin (1) and hypophyllanthin (2), the important lignans of Phyllanthus species, especially Phyllanthus amarus. Separation of 1 and 2 was carried out on silica gel 60 F₂₅₄ la...

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Bibliographic Details
Published in:Phytochemical analysis 2006-11, Vol.17 (6), p.394-397
Main Authors: Tripathi, A.K, Verma, R.K, Gupta, A.K, Gupta, M.M, Khanuja, S.P.S
Format: Article
Language:English
Subjects:
alkaloids
chemical analysis
Chromatography, Thin Layer - methods
Euphorbiaceae
HPLC
HPTLC
hypophyllanthin
lignans
Lignans - chemistry
medicinal plants
phyllanthin
Phyllanthus
Phyllanthus - chemistry
Phyllanthus amarus
Phyllanthus debilis
Phyllanthus fraternus
Phyllanthus maderaspatensis
Phyllanthus species
Phyllanthus urinaria
Phyllanthus virgatus
quantitative analysis
quantitative determination
thin layer chromatography
traditional medicine
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Summary:A simple, precise and rapid high-performance thin-layer chromatographic method has been developed for the estimation of phyllanthin (1) and hypophyllanthin (2), the important lignans of Phyllanthus species, especially Phyllanthus amarus. Separation of 1 and 2 was carried out on silica gel 60 F₂₅₄ layers eluted with hexane:acetone:ethyl acetate (74:12:8), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid and ethanol. Scanning and quantification of spots was performed at 580 nm. Recoveries of 1 and 2 were 98.7 and 97.3%, respectively. The method was validated and the peak purities and limits of detection and quantification were determined.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.936