Simultaneous determination of 11 characteristic components in three species of Curcuma rhizomes using pressurized liquid extraction and high-performance liquid chromatography

A high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and pressurized liquid extraction was developed for simultaneous quantitative determination of 11 characteristic compounds, including curcumenone, curcumenol, neocurdione, curdione, isocurcumenol, furanodienone, c...

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Bibliographic Details
Published in:Journal of Chromatography A 2006-11, Vol.1134 (1), p.226-231
Main Authors: Yang, F.Q., Wang, Y.T., Li, S.P.
Format: Article
Language:English
Subjects:
Acetonitriles - metabolism
Aroma and flavouring agent industries
Biological and medical sciences
Calibration
Chromatography, Gas
Chromatography, High Pressure Liquid
Curcuma
Curcuma - chemistry
Curcuma - classification
Food industries
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC-DAD
Mass Spectrometry
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - analysis
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Pressurized liquid extraction
Quality control
Regression Analysis
Rhizome - chemistry
Sesquiterpenoids
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Summary:A high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and pressurized liquid extraction was developed for simultaneous quantitative determination of 11 characteristic compounds, including curcumenone, curcumenol, neocurdione, curdione, isocurcumenol, furanodienone, curcumol, germacrone, curzerene, furanodiene and β-elemene, in rhizomes of three species of Curcuma. The analysis was performed on an ODS C 18 column. The mobile phase consisted of (A) water and (B) acetonitrile using a gradient elution. The peaks were monitored at both 214 nm and 256 nm. All calibration curves showed good linearity ( r 2 > 0.9996) within test ranges. This method showed good repeatability for the quantification of these eleven components in three species Curcuma rhizomes with intra- and inter-day variations of less than 1.57% and 1.98%, respectively. The validated method was successfully applied to quantify 11 investigated components in eighteen samples of three species of Curcuma, which is helpful to control their quality.
ISSN:0021-9673
DOI:10.1016/j.chroma.2006.09.048