Tissue engineering with electric fields: Immobilization of mammalian cells in multilayer aggregates using dielectrophoresis

Positive dielectrophoresis can be used to create aggregates of animal cells with 3D architectures. It is shown that the cells, when pulled together into an aggregate by positive dielectrophoresis in a low-conductivity iso-osmotic solution, adhere to each other. The adherence of the cells to each oth...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2007-10, Vol.98 (3), p.694-700
Main Authors: Sebastian, Anil, Buckle, Anne-Marie, Markx, Gerard H
Format: Article
Language:English
Subjects:
3D culture
adhesion
Biological and medical sciences
Biotechnology
Cell adhesion & migration
Cell Aggregation - drug effects
Cell Aggregation - physiology
Cell Culture Techniques - methods
Cell Separation - methods
Cells
dielectrophoresis
Electromagnetic Fields
Electrophoresis - methods
Fundamental and applied biological sciences. Psychology
Humans
immobilization
Jurkat Cells
Mammals
Tissue engineering
Tissue Engineering - methods
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Summary:Positive dielectrophoresis can be used to create aggregates of animal cells with 3D architectures. It is shown that the cells, when pulled together into an aggregate by positive dielectrophoresis in a low-conductivity iso-osmotic solution, adhere to each other. The adherence of the cells to each other is non-specific and increases in time, and after 10-15 min becomes strong enough to immobilize the cells in the aggregate, enabling the ac electric field to be released, and the iso-osmotic buffer to be replaced by growth or other media. Cell viability is maintained. The new method of immobilization significantly simplifies the construction of aggregates of animal cells by dielectrophoresis, and increases the utility of dielectrophoresis in tissue engineering and related areas. Biotechnol. Bioeng. 2007;98: 694-700. © 2007 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.21416