Development of Y Chromosome Intraspecific Polymorphic Markers in the Felidae

Y chromosome haplotyping based on microsatellites and single nucleotide polymorphisms (SNPs) has proved to be a powerful tool for population genetic studies of humans. However, the promise of the approach is hampered in the majority of nonhuman mammals by the lack of Y-specific polymorphic markers....

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Published in:The Journal of heredity 2007-01, Vol.98 (5), p.400-413
Main Authors: Luo, Shu-Jin, Johnson, Warren E, David, Victor A, Menotti-Raymond, Marilyn, Stanyon, Roscoe, Cai, Qing Xiu, Beck, Thomas, Yuhki, Naoya, Pecon-Slattery, Jill, Smith, James L.D, O'Brien, Stephen J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cats
Cats - genetics
Chromosome Mapping
Chromosomes
DNA Primers
Felidae
Felidae - genetics
Felis catus
Genetic markers
In Situ Hybridization, Fluorescence
Male
Males
Microsatellite Repeats
Molecular biology
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Population genetics
Y Chromosome
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Summary:Y chromosome haplotyping based on microsatellites and single nucleotide polymorphisms (SNPs) has proved to be a powerful tool for population genetic studies of humans. However, the promise of the approach is hampered in the majority of nonhuman mammals by the lack of Y-specific polymorphic markers. We were able to identify new male-specific polymorphisms in the domestic cat Felis catus and 6 additional Felidae species with a combination of molecular genetic and cytogenetic approaches including 1) identifying domestic cat male-specific microsatellites from markers generated from a male cat microsatellite-enriched genomic library, a flow-sorted Y cosmid library, or a Y-specific cat bacteria artificial chromosome (BAC) clone, (2) constructing microsatellite-enriched libraries from flow-sorted Y chromosomes isolated directly from focal wildcat species, and (3) screening Y chromosome conserved anchored tagged sequences primers in Felidae species. Forty-one male-specific microsatellites were identified, but only 6 were single-copy loci, consistent with the repetitive nature of the Y chromosome. Nucleotide diversity (π) of Y-linked intron sequences (2.1 kbp) was in the range of 0 (tiger) to 9.95 × 10−4 (marbled cat), and the number of SNPs ranged from none in the tiger to 7 in the Asian leopard cat. The Y haplotyping system described here, consisting of 4 introns (SMCY3, SMCY7, UTY11, and DBY7) and 1 polymorphic microsatellite (SMCY-STR), represents the first available markers for tracking intraspecific male lineage polymorphisms in Felidae species and promises to provide significant insights to evolutionary and population genetic studies of the species.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/esm063