Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction

eer4 was isolated as an Arabidopsis mutant with an extreme response to ethylene in dark-grown seedlings that was also found to have partial ethylene insensitivity at the level of ethylene-dependent gene expression, including ERF1. Subsequent cloning of eer4 revealed an inappropriate stop codon in a...

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Bibliographic Details
Published in:Journal of experimental botany 2007-01, Vol.58 (10), p.2627-2639
Main Authors: Robles, Linda M, Wampole, Jessica S, Christians, Matthew J, Larsen, Paul B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis
Arabidopsis - drug effects
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis Proteins - physiology
Codon, Nonsense
Darkness
EER4
ethylene
Ethylenes - metabolism
Ethylenes - pharmacology
Gene expression
Genes
Genetic mutation
Green Fluorescent Proteins - analysis
Hypocotyls
Models, Biological
Molecular Sequence Data
Peptide Termination Factors - metabolism
Phenotype
Phenotypes
Receptors
Research Papers
RNA
Seedlings
Sequence Analysis, Protein
Signal Transduction - genetics
Taf12b
Transcription Factor TFIID - genetics
Transcription Factor TFIID - metabolism
Transcription Factor TFIID - physiology
Transcription factors
triple response
Yeasts
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Summary:eer4 was isolated as an Arabidopsis mutant with an extreme response to ethylene in dark-grown seedlings that was also found to have partial ethylene insensitivity at the level of ethylene-dependent gene expression, including ERF1. Subsequent cloning of eer4 revealed an inappropriate stop codon in a previously uncharacterized TFIID-interacting transcription factor homologous to human TAF12 and yeast TAF61. Genetic and pharmacological analysis demonstrated that the eer4 phenotype is strictly ethylene dependent in seedlings, yet a double mutant with the partially ethylene-insensitive Arabidopsis mutant, ein3-1, had restored ethylene responsiveness, indicating that eer4 also regulates a previously unknown resetting or dampening mechanism for the ethylene signalling pathway. Consistent with the absolute requirement of EER4 for ERF1 expression, biochemical analysis showed that EER4 is localized to the nucleus where it probably recruits EIN3 and probably other transcription factors along with components of the TFIID complex for expression of a subset of genes required for either manifestation or subsequent dampening of the response to ethylene.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/erm080