Myeloid Leukemia Factor 1 Associates with a Novel Heterogeneous Nuclear Ribonucleoprotein U-like Molecule

Myeloid leukemia factor 1 (MLF1) is an oncoprotein associated with hemopoietic lineage commitment and acute myeloid leukemia. Here we show that Mlf1 associated with a novel binding partner, Mlf1-associated nuclear protein (Manp), a new heterogenous nuclear ribonucleoprotein (hnRNP) family member, re...

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Bibliographic Details
Published in:The Journal of biological chemistry 2006-12, Vol.281 (50), p.38791-38800
Main Authors: Winteringham, Louise N., Endersby, Raelene, Kobelke, Simon, McCulloch, Ross K., Williams, James H., Stillitano, Justin, Cornwall, Scott M., Ingley, Evan, Klinken, S.Peter
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cell Cycle Proteins
Cell Nucleus - metabolism
Chlorocebus aethiops
COS Cells
DNA, Complementary
DNA-Binding Proteins
Heterogeneous-Nuclear Ribonucleoprotein U - metabolism
Molecular Sequence Data
Protein Binding
Proteins - chemistry
Proteins - genetics
Proteins - metabolism
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Summary:Myeloid leukemia factor 1 (MLF1) is an oncoprotein associated with hemopoietic lineage commitment and acute myeloid leukemia. Here we show that Mlf1 associated with a novel binding partner, Mlf1-associated nuclear protein (Manp), a new heterogenous nuclear ribonucleoprotein (hnRNP) family member, related to hnRNP-U. Manp localized exclusively in the nucleus and could redirect Mlf1 from the cytoplasm into the nucleus. The nuclear content of Mlf1 was also regulated by 14-3-3 binding to a canonical 14-3-3 binding motif within the N terminus of Mlf1. Significantly Mlf1 contains a functional nuclear export signal and localized primarily to the nuclei of hemopoietic cells. Mlf1 was capable of binding DNA, and microarray analysis revealed that it affected the expression of several genes, including transcription factors. In summary, this study reveals that Mlf1 translocates between nucleus and cytoplasm, associates with a novel hnRNP, and influences gene expression.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M605401200