Identification of a Novel Isoform of Microsomal Triglyceride Transfer Protein

Microsomal triglyceride transfer protein (MTP) has been studied extensively, primarily because of its role in the assembly of very low density lipoproteins by the liver and chylomicrons by the intestine. Recent studies have suggested that MTP may also play key roles in other cellular processes. In t...

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Bibliographic Details
Published in:The Journal of biological chemistry 2007-09, Vol.282 (37), p.26981-26988
Main Authors: Mohler, Peter J., Zhu, Mei-Ying, Blade, Anna M., Ham, Amy-Joan L., Shelness, Gregory S., Swift, Larry L.
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Amino Acid Sequence
Animals
Carrier Proteins - chemistry
Carrier Proteins - genetics
Carrier Proteins - isolation & purification
Carrier Proteins - physiology
Chlorocebus aethiops
COS Cells
Mass Spectrometry
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Protein Isoforms
Transfection
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Summary:Microsomal triglyceride transfer protein (MTP) has been studied extensively, primarily because of its role in the assembly of very low density lipoproteins by the liver and chylomicrons by the intestine. Recent studies have suggested that MTP may also play key roles in other cellular processes. In this paper we report the identification of a novel splice variant of MTP in mice. This isoform, MTP-B, has a unique first exon located ∼2.7 kilobases upstream of canonical MTP (MTP-A) exon 1. The alternative exon encodes 35 amino acids compared with 20 amino acids encoded by exon 1 of MTP-A. MTP-B represents ∼90% of total MTP mRNA in mouse adipocytes and 3T3-L1 cells and
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M700500200