Intraperitoneal Injection of Oxygenated Perfluorochemical Improves the Outcome of Intraportal Islet Transplantation in a Rat Model

A 50% to 75% early graft loss upon engraftment has been suggested to in intraportal islet transplantation (IPIT). Hypoxia in the portal vein contributes to graft loss in immediately posttransplantation. Herein we examined the effect on the outcome of IPIT of intraperitoneal oxygenated perfluorochemi...

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Bibliographic Details
Published in:Transplantation proceedings 2006-12, Vol.38 (10), p.3289-3292
Main Authors: Sakai, T., Li, S., Tanioka, Y., Goto, T., Tanaka, T., Matsumoto, I., Tsujimura, T., Fujino, Y., Suzuki, Y., Kuroda, Y.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood Glucose - metabolism
Diabetes Mellitus, Experimental - surgery
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Fluorocarbons - therapeutic use
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Graft Survival - drug effects
Injections, Intraperitoneal
Islets of Langerhans Transplantation - methods
Islets of Langerhans Transplantation - physiology
Medical sciences
Models, Animal
Oxygen - administration & dosage
Portal System
Rats
Rats, Inbred Lew
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue, organ and graft immunology
Transplantation, Isogeneic
Treatment Outcome
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Summary:A 50% to 75% early graft loss upon engraftment has been suggested to in intraportal islet transplantation (IPIT). Hypoxia in the portal vein contributes to graft loss in immediately posttransplantation. Herein we examined the effect on the outcome of IPIT of intraperitoneal oxygenated perfluorochemical (PFC) as an oxygen carrier. Isolated Lewis rat islets were transplanted into the portal vein of a chemically induced diabetic syngeneic rat. First, 1500 IEQ was determined to be the optimal dose in this study. When oxygenated PFC (group 1) was intraperitoneally injected following IPIT of 1500 IEQ, the success rate of transplantation was 5/6, in contrast to 1/6 when PFC with no oxygen was injected (group 2) and 1/6 in IPIT without PFC, respectively. The area under the glucose profile curve on intraperitoneal glucose tolerance tests on posttransplant day 28 in group 1 was significantly smaller than that for group 2. In conclusion, intraperitoneal oxygenated PFC improved the outcome of IPIT.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2006.10.183