Monitoring Single-Cell Infectivity from Virus-Particle Nanoarrays Fabricated by Parallel Dip-Pen Nanolithography

Sting like a bee: Nanoarrays of infectious virus particles, encoded with EGFP, are patterned by dip‐pen nanolithography and exposed to a solution of cells. Upon infection, infected cells produce the EGFP protein, generating a green fluorescence signal that allows one to monitor the cellular infectio...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2007-09, Vol.3 (9), p.1482-1485
Main Authors: Vega, Rafael A., Shen, Clifton K.-F., Maspoch, Daniel, Robach, Jessica G., Lamb, Robert A., Mirkin, Chad A.
Format: Article
Language:English
Subjects:
Animals
Biological Assay - instrumentation
Biological Assay - methods
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
cell infection
Cell Line
Cercopithecus aethiops
dip-pen nanolithography
Equipment Design
Equipment Failure Analysis
Kidney - virology
Microarray Analysis - instrumentation
Microarray Analysis - methods
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology - instrumentation
Nanotechnology - methods
patterning
templating
Virion - physiology
viruses
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Summary:Sting like a bee: Nanoarrays of infectious virus particles, encoded with EGFP, are patterned by dip‐pen nanolithography and exposed to a solution of cells. Upon infection, infected cells produce the EGFP protein, generating a green fluorescence signal that allows one to monitor the cellular infection process in real time (as seen in the optical image). These results suggest that antibody‐immobilized virus particles retain their biological activity. Scale bar: 35μm.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200700244