Effect of Immature Dendritic Cell Injection Before Heterotropic Cardiac Allograft

Although dendritic cells (DCs) are unrivaled for initiation of immune responses, the immunomodulatory capacity of chemically fixed DC has not been thoroughly evaluated. We monitored the tolerogenic capacity of chemically fixed DCs using allogeneic heart transplantations. Bone marrow progenitors were...

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Bibliographic Details
Published in:Transplantation proceedings 2006-12, Vol.38 (10), p.3189-3192
Main Authors: Oh, B.C., Lee, H.M., Lim, D.P., Cho, J.J., Lee, G., Lee, D.S., Lee, J.R.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biological and medical sciences
Cell Culture Techniques
Cell Separation
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - transplantation
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Heart Transplantation - immunology
Interleukin-12 - deficiency
Interleukin-12 - genetics
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Medical sciences
Mice
Mice, Inbred C57BL
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue, organ and graft immunology
Transplantation, Heterotopic
Transplantation, Homologous - immunology
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Summary:Although dendritic cells (DCs) are unrivaled for initiation of immune responses, the immunomodulatory capacity of chemically fixed DC has not been thoroughly evaluated. We monitored the tolerogenic capacity of chemically fixed DCs using allogeneic heart transplantations. Bone marrow progenitors were differentiated into immature DCs which were then chemically fixed and injected intravenously into recipient mice at 14 days before allogeneic heart transplantation. Chemically fixed DCs markedly prolonged graft survival in the major histocompatibility complex (MHC) I/II mismatch cardiac transplantation (B6 → B10.A; median survival time [MST] 12.5 days vs >70 days). T cells that encountered chemically fixed DCs showed attenuated apoptotic cell death and inactivated phenotypes after allogeneic heterotropic heart transplantation. Furthermore, when DCs from interleukin (IL)-10 −/− mice were treated, the in vitro T-cell response was greater than that from IL-12 −/− mice. We have suggested that the chemically fixed DCs may mediate peripheral T-cell tolerance, with therapeutic potential for allogeneic transplantation.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2006.10.180