Laser-assisted hatching of cryopreserved—thawed embryos by thinning one quarter of the zona

Laser-assisted hatching is little documented in the literature regarding its efficacy in cryopreserved—thawed (CT) embryo transfer cycles. The aim of the present study was to evaluate in a randomized manner the efficacy of thinning one quarter of the zona pellucida of CT embryos to a depth of 50–80%...

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Bibliographic Details
Published in:Reproductive biomedicine online 2006-11, Vol.13 (5), p.668-675
Main Authors: Petersen, Claudia Guilhermino, Mauri, Ana Lucia, Baruffi, Ricardo Luiz Razera, Oliveira, João Batista Alcãntara, Felipe, Valeria, Massaro, Fabiana Cagnoto, Franco, Jose Gonçalves
Format: Article
Language:English
Subjects:
Adult
Cryopreservation - methods
cryopreserved–thawed embryos
Embryo Implantation
Embryo Transfer
Female
Humans
implantation
Laser Therapy
Pregnancy
quarter laser zona thinning
Sperm Injections, Intracytoplasmic - methods
Zona Pellucida - ultrastructure
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Summary:Laser-assisted hatching is little documented in the literature regarding its efficacy in cryopreserved—thawed (CT) embryo transfer cycles. The aim of the present study was to evaluate in a randomized manner the efficacy of thinning one quarter of the zona pellucida of CT embryos to a depth of 50–80% of the original thickness, via laser treatment (the qLZT-AH procedure), in improving implantation and pregnancy rates. Two populations were studied: population I, patients who had all their supernumerary embryos cryopreserved, regardless of their morphology, and population II, patients at risk of ovarian hyperstimulation syndrome who had all their embryos cryopreserved. Artificial and natural protocols were used for the embryo transfers. A total of 350 laser-thinned CT embryos were compared with 352 intact zona embryos. No difference in implantation or pregnancy rate was found after using qLZT-AH in either population. These findings suggest that qLZT-AH should not be routinely performed in cryopreserved embryo programmes.
ISSN:1472-6483
1472-6491
DOI:10.1016/S1472-6483(10)60657-8