Differential expression of dehydrin in flower buds of two Japanese apricot cultivars requiring different chilling requirements for bud break

In this study, we investigated seasonal changes in protein profiles in dormant flower buds of Japanese apricot (Prunus mume Siebold Zucc.) cultivars 'Ellching', from subtropical Taiwan, and 'Nanko', from temperate Japan. One protein, isolated by two-dimensional polyacrylamide gel...

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Bibliographic Details
Published in:Tree physiology 2006-12, Vol.26 (12), p.1559-1563
Main Authors: Yamane, H, Kashiwa, Y, Kakehi, E, Yonemori, K, Mori, H, Hayashi, K, Iwamoto, K, Tao, R, Kataoka, I
Format: Article
Language:English
Subjects:
buds
chilling requirement
Cold Temperature
cultivars
dehydrin
dormancy breaking
Electrophoresis, Gel, Two-Dimensional
flowers
Flowers - growth & development
Flowers - metabolism
gene expression
genetic variation
late embryogenesis-abundant proteins
Plant Proteins - biosynthesis
protein composition
protein synthesis
Prunus - growth & development
Prunus - metabolism
Prunus mume
seasonal variation
transcription (genetics)
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Summary:In this study, we investigated seasonal changes in protein profiles in dormant flower buds of Japanese apricot (Prunus mume Siebold Zucc.) cultivars 'Ellching', from subtropical Taiwan, and 'Nanko', from temperate Japan. One protein, isolated by two-dimensional polyacrylamide gel electrophoresis of flower bud extracts, was shown by peptide sequencing to be a dehydrin (the group of D-11 LEA (late embryogenesis-abundant) proteins). Patterns of dehydrin protein and transcript accumulation differed between the cultivars, with greater accumulations and longer persistence in 'Nanko' than in 'Ellching'. These differences correspond with the greater requirement for chilling to break flower bud dormancy in 'Nanko' than in 'Ellching'. Our study supports the findings of earlier work comparing dehydrin expression in the bark tissue of the evergreen and deciduous peach (Prunus persica (L.) Batsch) genotypes, and suggests that the role of dehydrin during the dormant season is common to all Prunus species.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/26.12.1559