Purified allergens vs. complete extract in the diagnosis of plane tree pollen allergy

Summary Background Plane tree pollen allergy is a clinical disorder affecting human population in cities of Europe, North America, South Africa, and Australia. Objective To compare IgE‐reactivity of the natural and recombinant forms of two major plane allergens, Pla a 1 and Pla a 2, with the reactiv...

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Bibliographic Details
Published in:Clinical and experimental allergy 2006-12, Vol.36 (12), p.1505-1512
Main Authors: Asturias, J. A., Ibarrola, I., Amat, P., Tella, R., Malet, A., Cisteró-Bahíma, A., Enrique, E., Malek, T., Martínez, A.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Allergens - isolation & purification
Allergic diseases
Antigens, Plant
Biological and medical sciences
Bioreactors
Case-Control Studies
Child
component-resolved diagnosis
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
IgE detection
Immunoblotting
Immunoglobulin E - blood
Immunopathology
Intradermal Tests - methods
Male
Medical sciences
Middle Aged
Pla a 1
Pla a 2
plane tree allergy
Plant Extracts
Pollen
Radioallergosorbent Test
recombinant allergen
Recombinant Proteins - isolation & purification
Rhinitis, Allergic, Seasonal - diagnosis
Sensitivity and Specificity
skin prick tests
Statistics, Nonparametric
Trees
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Summary:Summary Background Plane tree pollen allergy is a clinical disorder affecting human population in cities of Europe, North America, South Africa, and Australia. Objective To compare IgE‐reactivity of the natural and recombinant forms of two major plane allergens, Pla a 1 and Pla a 2, with the reactivity of Platanus acerifolia pollen extract. Methods Forty‐seven patients with P. acerifolia allergy, 15 of them monosensitized, and 24 control subjects were included in the study. Natural Pla a 1 and Pla a 2 were purified by standard chromatographic methods and recombinant proteins were expressed in Escherichia coli. Skin prick test and determination of specific IgE were performed with commercial P. acerifolia extract and natural and recombinant purified allergens. Results Pla a 1 and Pla a 2 were responsible for 79% of the IgE‐binding capacity against P. acerifolia pollen extract. A high correlation has been found between the IgE response to nPla a 1 (R=0.80; P
ISSN:0954-7894
1365-2222
DOI:10.1111/j.1365-2222.2006.02591.x