Hybrid titania-aminosilane platforms evaluated with human mesenchymal stem cells

The properties of hybrid aminopropyltriethoxysilane–tetraisopropylorthotitanate (APTS‐TIPT) platforms prepared by a sol–gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be...

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Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2007-10, Vol.83B (1), p.232-239
Main Authors: Manso-Silván, M., Rodríguez-Navas, C., Arroyo-Hernández, M., López-Elvira, E., Gago, R., Vázquez, L., Agulló-Rueda, F., Climent, A., Martínez-Duart, J. M., García-Ruiz, J. P.
Format: Article
Language:English
Subjects:
aminosilane
Biocompatible Materials - chemistry
Biocompatible Materials - metabolism
Cells, Cultured
Humans
hybrid materials
Hydrogen-Ion Concentration
Imines - chemistry
Materials Testing
mesenchymal stem cell
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Silanes - chemistry
Silanes - metabolism
sol-gel
Titanium - chemistry
Titanium - metabolism
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Summary:The properties of hybrid aminopropyltriethoxysilane–tetraisopropylorthotitanate (APTS‐TIPT) platforms prepared by a sol–gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be preferably surface‐oriented as indicated by microanalytical techniques. Furthermore, the surface showed characteristic amino‐silane bands when explored by Raman spectroscopy as well as indications of silane and titanate condensation. Surface activity of the amino groups was probed by ultraviolet–visible spectroscopy imine derivatization and chemical force spectroscopy, showing a pH‐dependent surface charge‐induced potential. hMSCs cultured onto these surfaces showed relevant differences with respect to their behavior on gelatin‐coated glass plates. Even if with a lower proliferative rate than controls, the cells develop long cytosolic prolongations in osteogenic differentiation medium, thus, supporting the idea of an APTS‐TIPT stimulated process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.30788