BRG1 Chromatin Remodeling Activity Is Required for Efficient Chromatin Binding by Repressor Element 1-silencing Transcription Factor (REST) and Facilitates REST-mediated Repression

Chromatin remodeling enzymes such as SWI/SNF use the hydrolysis of ATP to power the movement of nucleosomes with respect to DNA. BRG1, one of the ATPases of the SWI/SNF complex, can be recruited by both activators and repressors, although the precise role of BRG1 in mechanisms of repression has thus...

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Bibliographic Details
Published in:The Journal of biological chemistry 2006-12, Vol.281 (51), p.38974-38980
Main Authors: Ooi, Lezanne, Belyaev, Nikolai D., Miyake, Katsuhide, Wood, Ian C., Buckley, Noel J.
Format: Article
Language:English
Subjects:
Acetylation
Adenosine Triphosphate - chemistry
Binding Sites
Cell Line
Cell Nucleus - metabolism
Chromatin - chemistry
DNA Helicases - chemistry
DNA Helicases - physiology
Histones - chemistry
Humans
Hydroxamic Acids - pharmacology
Nuclear Proteins - chemistry
Nuclear Proteins - physiology
Plasmids - metabolism
Protein Binding
Repressor Proteins - chemistry
Repressor Proteins - physiology
Transcription Factors - chemistry
Transcription Factors - physiology
Transcription, Genetic
Transfection
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Summary:Chromatin remodeling enzymes such as SWI/SNF use the hydrolysis of ATP to power the movement of nucleosomes with respect to DNA. BRG1, one of the ATPases of the SWI/SNF complex, can be recruited by both activators and repressors, although the precise role of BRG1 in mechanisms of repression has thus far remained unclear. One transcription factor that recruits BRG1 as a corepressor is the repressor element 1-silencing transcription factor (REST). Here we address for the first time the mechanism of BRG1 activity in gene repression. We found that BRG1 enhanced REST-mediated repression at some REST target genes by increasing the interaction of REST with the local chromatin at its binding sites. Furthermore, REST-chromatin interactions, mediated by BRG1, were enhanced following an increase in histone acetylation in a manner dependent on the BRG1 bromodomain. Our data suggest that BRG1 facilitates REST repression by increasing the interaction between REST and chromatin. Such a mechanism may be applicable to other transcriptional repressors that utilize BRG1.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M605370200