STEM tomography in cell biology

Transmission electron tomography has been used in biological sciences for quite some time and proven to be a valuable tool. However, to date, the different Scanning Transmission modes are almost not used for electron tomography on resin-embedded biological material. We explored different STEM modes...

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Bibliographic Details
Published in:Journal of structural biology 2007-09, Vol.159 (3), p.381-391
Main Authors: Yakushevska, A.E., Lebbink, M.N., Geerts, W.J.C., Spek, L., van Donselaar, E.G., Jansen, K.A., Humbel, B.M., Post, J.A., Verkleij, A.J., Koster, A.J.
Format: Article
Language:English
Subjects:
Bright Field-TEM
Cell Membrane - ultrastructure
Cells - ultrastructure
Electron tomography
HAADF-STEM
Humans
Microscopy, Electron, Scanning Transmission - methods
Template matching
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Summary:Transmission electron tomography has been used in biological sciences for quite some time and proven to be a valuable tool. However, to date, the different Scanning Transmission modes are almost not used for electron tomography on resin-embedded biological material. We explored different STEM modes on epon-embedded, osmium–uranyl-lead-stained biological material. Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms from the same areas were recorded and compared. Contrast and signal-to-noise ratios were calculated. Template matching was used to validate results obtained in Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms. It is concluded that High Angle Annular Dark Field-STEM gives a five times better contrast and signal-to-noise ratio than Bright Field-TEM. Template matching showed that 1.3 times more information could be extracted from High Angle Annular Dark Field-STEM tomograms than from Bright Field-TEM tomograms.
ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2007.04.006