Prospective identification of myogenic endothelial cells in human skeletal muscle

We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells reg...

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Bibliographic Details
Published in:Nature biotechnology 2007-09, Vol.25 (9), p.1025-1034
Main Authors: Cassino, Theresa, Li, Guangheng, Gharaibeh, Burhan, Cao, Baohong, Deasy, Bridget M, Sun, Bin, Huard, Johnny, Péault, Bruno, Pollett, Jonathan B, Crisan, Mihaela, Logar, Alison, Drowley, Lauren, Yap, Solomon, Zheng, Bo
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Agriculture
Animals
Bioinformatics
Biological and medical sciences
Biomarkers - metabolism
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
CD56 Antigen
Cell culture
Cell Proliferation
Cell Survival
Cells, Cultured
Cellular biology
Child
Clone Cells
Endothelial Cells - cytology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Histology
Humans
Industrial applications and implications. Economical aspects
Karyotypes
Life Sciences
Mice
Mice, SCID
Middle Aged
Miscellaneous
Muscle, Skeletal - cytology
Muscle, Skeletal - physiology
Musculoskeletal system
Neoplasms - pathology
Oxidative stress
Regeneration
Rodents
Time Factors
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Description
Summary:We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56 + myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56 + myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro , and may have potential for the treatment of human muscle disease.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt1334