Genetic diversity in wild (Sus scrofa scrofa) and domestic (Sus scrofa domestica) pigs and their hybrids based on polymorphism of a fragment of the D-loop region in the mitochondrial DNA

We examined the variation in mitochondrial DNA by sequencing the D-loop region in wild and domestic (large-white breed) pigs, in hybrids between domestic and wild pigs, and in Monteiro pigs. A D-loop fragment of approximately 330 bp was amplified by PCR. Sequencing of DNA amplicons identified haplot...

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Bibliographic Details
Published in:Genetics and molecular research 2006-01, Vol.5 (4), p.564-568
Main Authors: Grossi, S.F, Lui, J.F, Garcia, J.E, Meirelles, F.V
Format: Article
Language:English
Subjects:
Animals
Animals, Wild
Base Sequence
DNA, Mitochondrial - analysis
domestic animals
evolution
genetic polymorphism
genetic variation
Genetic Variation - genetics
Haplotypes
hybrids
mitochondrial DNA
molecular genetics
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic - genetics
provenance
sequence analysis
Sus scrofa
Sus scrofa - classification
Sus scrofa - genetics
swine
wild animals
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Summary:We examined the variation in mitochondrial DNA by sequencing the D-loop region in wild and domestic (large-white breed) pigs, in hybrids between domestic and wild pigs, and in Monteiro pigs. A D-loop fragment of approximately 330 bp was amplified by PCR. Sequencing of DNA amplicons identified haplotypes previously described as European and Asian types. Monteiro pigs and wild pigs had European haplotypes and domestic pigs had both European and Asian haplotypes.
ISSN:1676-5680
1676-5680