Effect of conjugated linoleic acid type, treatment period, and dosage on differentiation of 3T3 cells

• Published in: Lipids 2006-10, Vol.41 (10), p.937-949
• Main Authors: He, M. L, Hnin, T. M, Kuwayama, H, Mir, P. S, Okine, E. K, Hidari, H
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Animals; Cell differentiation; Cell Differentiation - drug effects; conjugated linoleic acid; cultured cells; Dose-Response Relationship, Drug; enzyme activity; fatty acids; Fatty Acids - analysis; glycerol-3-phosphate dehydrogenase; Glycerolphosphate Dehydrogenase - metabolism; linoleic acid; Linoleic Acid - pharmacology; Linoleic Acids, Conjugated - pharmacology; lipid content; Mice; Time Factors
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-006-5047-0

This study was conducted to determine effect of CLA and linoleic acid (LA) on cell differentiation, cellular glycerol-3-phosphate dehydrogenase (GPDH) activity, and FA accumulation in differentiating 3T3-L1 cells (3 isomersx3 treatment periodsx4 doses). The cells were cultured in 24-well plates for proliferation until confluence. Then they were treated with media containing 0, 10, 35, or 70 mg/L (0, 35, 125, or 250 mmol/L, respectively) of LA,cis9,trans11-ortrans10,cis12-CLA during early (day 0-2), intermediate and late (day 3-8), or overall (day 0-8) differentiation periods. Dexamethasone, methyl-isobutylxanthine, and insulin were supplemented to the media only for the early period to induce the differentiation. On day 8 of postconfluence the cells were harvested for Oil Red O staining, analysis of GPDH activity, and determination of the FA concentration. Cellular LA or CLA was found to accumulate in a dose-response manner, mainly during the intermediate/late period. Treatment withtrans10,cis12-CLA lowered (P