Species-Diagnostic Single-Nucleotide Polymorphism and Sequence-Tagged Site Markers for the Parasitic Wasp Genus Nasonia (Hymenoptera: Pteromalidae)

Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for...

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Bibliographic Details
Published in:Journal of economic entomology 2007-08, Vol.100 (4), p.1033-1036
Main Authors: Niehuis, O., Judson, A. K., Werren, J. H., Hunter, W. B., Dang, P. M., Dowd, S. E., Grillenberger, B., Beukeboom, L. W., Gadau, J.
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
Ecotilling
Ecotilling technique
expressed sequence tags
Genetic Markers
genetic variation
genome mapping
molecular systematics
Nasonia
Nasonia giraulti
Nasonia vitripennis
nucleotide sequences
parasitoid identification
parasitoids
Polymorphism, Single Nucleotide
RAPID COMMUNICATION
Sequence Tagged Sites
single nucleotide polymorphism
species differences
species identification
Species Specificity
taxonomy
Wasps - classification
Wasps - genetics
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Summary:Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.
ISSN:0022-0493
1938-291X
DOI:10.1603/0022-0493%282007%29100%5B1033%3ASSPASS%5D2.0.CO%3B2