Basis of beneficial immunomodulation by monoclonal antibodies against Streptococcus mutans adhesin P1

We previously identified five monoclonal antibodies (MAbs) against Streptococcus mutans adhesin P1 that modulate the humoral response when bound to whole bacteria and immune complexes (ICs) are administered to BALB/c mice. The two MAbs that redirected the response towards increased efficacy recogniz...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 2007-10, Vol.51 (1), p.102-111
Main Authors: Isoda, Ryutaro, Robinette, Rebekah A, Pinder, Trina L, McArthur, William P, Brady, L. Jeannine
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - immunology
Alanine
Animals
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - immunology
Antigen I/II family
Antigen-Antibody Complex - immunology
Antigen-antibody complexes
Applied microbiology
bacterial adhesin
Bacterial Adhesion
Bacteriology
Biological and medical sciences
Complement Activation
discontinuous epitopes
Epitopes
Female
Fundamental and applied biological sciences. Psychology
Immune response
Immune response (humoral)
Immune system
Immunization
Immunoglobulins
Immunomodulation
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Monoclonal antibodies
Phagocytes
Phagocytosis
Streptococcus infections
Streptococcus mutans
Streptococcus mutans - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:We previously identified five monoclonal antibodies (MAbs) against Streptococcus mutans adhesin P1 that modulate the humoral response when bound to whole bacteria and immune complexes (ICs) are administered to BALB/c mice. The two MAbs that redirected the response towards increased efficacy recognize discontinuous epitopes involving pre-alanine-rich domain sequence; therefore, to evaluate whether epitope specificity contributes to a desirable outcome a further MAb with this characteristic was tested. A beneficial immune response was promoted. None of the three MAbs that promoted a beneficial response was opsonic, suggesting that increased uptake of ICs by phagocytes does not mediate the improvement of the IC-elicited antibodies to inhibit bacterial adherence. Finally, two of the six anti-P1 MAbs activated complement but did not partition according to desirable vs. nondesirable effects.
ISSN:0928-8244
1574-695X
2049-632X
DOI:10.1111/j.1574-695X.2007.00279.x