role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagy

In the methanol-utilizing yeast Hansenula polymorpha, glucose and ethanol trigger the repression of peroxisomal enzymes at the transcriptional level, and rapid and selective degradation of methanol-induced peroxisomes by means of a process termed pexophagy. In this report we demonstrate that deficie...

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Bibliographic Details
Published in:FEMS yeast research 2007-10, Vol.7 (7), p.1103-1113
Main Authors: Stasyk, Olena G, van Zutphen, Tim, Ah Kang, Huyn, Stasyk, Oleh V, Veenhuis, Marten, Sibirny, Andriy A
Format: Article
Language:English
Subjects:
Alcohol oxidase
Alcohol Oxidoreductases - metabolism
Amino Acid Sequence
Carbohydrate Metabolism
Catabolite repression
Clonal deletion
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - physiology
Ethanol
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - physiology
Gene Deletion
Gene Expression Regulation, Fungal
Hansenula polymorpha
Methanol
Methanol - metabolism
Microscopy, Electron, Transmission
Microscopy, Immunoelectron
Models, Biological
Molecular Sequence Data
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - physiology
Peroxisomes
Peroxisomes - metabolism
Peroxisomes - ultrastructure
Pexophagy
Pichia - genetics
Pichia - metabolism
Pichia - ultrastructure
Repressor Proteins - chemistry
Repressor Proteins - genetics
Repressor Proteins - physiology
Repressors
Transcription factors
transcriptional repressors
yeast
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Summary:In the methanol-utilizing yeast Hansenula polymorpha, glucose and ethanol trigger the repression of peroxisomal enzymes at the transcriptional level, and rapid and selective degradation of methanol-induced peroxisomes by means of a process termed pexophagy. In this report we demonstrate that deficiency in the putative H. polymorpha homologues of transcriptional repressors Mig1 (HpMig1 and HpMig2), as well as HpTup1, partially and differentially affects the repression of peroxisomal alcohol oxidase by sugars and ethanol. As reported earlier, deficiency in HpTup1 leads to impairment of glucose- or ethanol-induced macropexophagy. In H. polymorpha mig1mig2 double-deletion cells, macropexophagy was also substantially impaired, whereas micropexophagy became a dominant mode of autophagic degradation. Our findings suggest that homologues of the elements of the Saccharomyces cerevisiae main repression pathway have pleiotropic functions in H. polymorpha.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2007.00286.x