Active Capture and Transport of Virus Particles Using a Biomolecular Motor-Driven, Nanoscale Antibody Sandwich Assay

Virus particles are captured and transported using kinesin‐driven, antibody‐functionalized microtubules. The functionalization was achieved through covalent crosslinking, which consequently enhanced the microtubule stability. The capture and transport of the virus particles was subsequently demonstr...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2006-03, Vol.2 (3), p.381-385
Main Authors: Bachand, George D., Rivera, Susan B., Carroll-Portillo, Amanda, Hess, Henry, Bachand, Marlene
Format: Article
Language:English
Subjects:
Antibodies - immunology
biomolecular motors
Equipment Design
Equipment Failure Analysis
Flow Injection Analysis - instrumentation
Flow Injection Analysis - methods
Immunoassay - instrumentation
Immunoassay - methods
kinesin
Kinesin - chemistry
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microtubules - chemistry
Molecular Motor Proteins - chemistry
Motion
nanodevices
nanofluidics
Nanotechnology - instrumentation
Nanotechnology - methods
transport
Virion - chemistry
Virion - immunology
Virion - isolation & purification
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Summary:Virus particles are captured and transported using kinesin‐driven, antibody‐functionalized microtubules. The functionalization was achieved through covalent crosslinking, which consequently enhanced the microtubule stability. The capture and transport of the virus particles was subsequently demonstrated in gliding motility assays in which antibody‐coated microtubules functioned as capture elements, and antibody‐coated microspheres served as fluorescent reporters (see Figure).
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200500262