Aberrant expression of Eag1 potassium channels in gastric cancer patients and cell lines

• Published in: Medical oncology (Northwood, London, England) London, England), 2007-01, Vol.24 (3), p.345-350
• Main Authors: Ding, Xiang-Wu, Luo, He-sheng, Jin, Xiong, Yan, Juan-juan, Ai, Yao-wei
• Format: Article
• Language: English
• Subjects: Adenoma - metabolism; Adult; Aged; Case-Control Studies; Cell Line, Tumor; Cell Proliferation; Chi-Square Distribution; Ether-A-Go-Go Potassium Channels - genetics; Ether-A-Go-Go Potassium Channels - metabolism; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Lymphatic Metastasis - diagnosis; Male; Matched-Pair Analysis; Middle Aged; Neoplasm Metastasis - physiopathology; Oncology; Reference Values; RNA, Messenger - analysis; Stomach Neoplasms - metabolism
• ISSN: 1357-0560; 1559-131X
• DOI: 10.1007/s12032-007-0015-y

Recently, an interesting relationship between potassium channels and cancer has evolved. The aim of this study is to investigate expression of Eag1 potassium channel in gastric cancer and its role in cancer cells growth. The expression of Eag1 for gasric cancer patients and cell lines as well as gastric adenoma was investigated by immunohistochemistry and reverse transcription polymerase chain reaction. In addition, imipramine was used to identify the involvement of Eag1 in the growth of SGC-7901 and BGC-823 cells. Frequency of positive expression of Eag1 protein was 70.5% (67/95) and Eag1 mRNA was 68.2% (15/22) in gastric cancer primary tissues. Eag1 mRNA was positively expressed in two gastric cell lines. Eag1 protein and mRNA were negatively expressed in paired non-cancerous matched tissues and 5 cases of adenoma tissues. The expression level of Eag1 protein was associated with lymph node metastasis (P = 0.049) and stage (P = 0.039), but had no correlation with sex, age, differentiation grades, and other organs metastases. Imipramine significantly inhibited the proliferation of SGC-7901 and BGC-823 cells at 12 h and 24 h detected by cells number counting and MTT assay (P < 0.01). The study indicates Eag1 is aberrantly expressed in gastric cancer tissues and cell lines and associated with cancer lymph node metastasis and stage and play an important role in the proliferation of gastric cancer cells.