Influence of low-dose polyunsaturated fatty acids supplementation on the inflammatory response of healthy adults

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2007-10, Vol.23 (10), p.724-730
• Main Authors: Schubert, Ralf, Ph.D, Kitz, Richard, M.D, Beermann, Christopher, Ph.D, Rose, Markus Andreas, M.D, Baer, Patrick Carolus, Ph.D, Zielen, Stefan, M.D, Boehles, Hansjosef, M.D
• Format: Article
• Language: English
• Subjects: adults; alpha-Linolenic Acid - blood; Alprostadil - blood; anti-inflammatory activity; arachidonic acid; Biological and medical sciences; Blood; blood lipids; Body mass index; Chromatography; Chronic illnesses; Cytokines; Dietary Supplements; Dinoprostone - blood; Disease; Double-Blind Method; Eicosanoic Acids - blood; eicosapentaenoic acid; Erythrocyte Membrane - chemistry; Fatty acids; Fatty Acids, Omega-3 - administration & dosage; Fatty Acids, Omega-3 - blood; Fatty Acids, Omega-3 - pharmacology; Fatty Acids, Omega-6 - administration & dosage; Fatty Acids, Omega-6 - blood; Fatty Acids, Omega-6 - pharmacology; Fatty Acids, Unsaturated - administration & dosage; Fatty Acids, Unsaturated - blood; Fatty Acids, Unsaturated - pharmacology; Feeding. Feeding behavior; Female; Fish oils; Fundamental and applied biological sciences. Psychology; gamma-linolenic acid; Gastroenterology and Hepatology; health promotion; human nutrition; Humans; Immune response; immunostimulants; Inflammation - blood; Inflammatory mediators; interleukin-10; Interleukin-10 - blood; interleukin-8; Interleukin-8 - blood; Leukotriene B4; leukotrienes; linolenic acid; Lipopolysaccharide; lipopolysaccharides; low fat diet; Male; Nutrition research; Polyunsaturated fatty acids; prostaglandins; stearic acid; Time Factors; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - blood; Vertebrates: anatomy and physiology, studies on body, several organs or systems; volunteers
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2007.06.012

Abstract Objective The aim of the present study was to examine the immune-modulating effect of two different fat blends enriched with a low dose of anti- or proinflammatory polyunsaturated fatty acids on the fatty acid status and subsequently on the immune response of healthy volunteers. Methods Thirty healthy volunteers were randomly assigned to group A (anti-inflammatory blend rich in polyunsaturated fatty acids: α-linolenic acid, 240 mg/d; eicosapentaenoic acid, 120 mg/d; stearidonic acid, 49 mg/d; and γ-linolenic acid, 73 mg/d) or group B (arachidonic acid, 40 mg/d; containing an inflammatory fat blend) for a 2-wk dietary supplementation period. Concentrations of interleukin-8, interleukin-10, tumor necrosis factor-α, prostaglandins E1 and E2 , and leukotriene B4 were investigated before, after 2 wk of supplementation, and 2 wk after stopping supplementation using a whole blood ex vivo lipopolysaccharide-stimulation assay. Results Plasma concentrations of α-linolenic acid and eicosapentaenoic acid were significantly increased in group A. In addition, dietary fat blends influenced eicosapentaenoic acid concentration in erythrocyte membranes. Supplementation of the fat blends resulted in contrasting effects on the expression of lipid mediators and cytokines after ex vivo lipopolysaccharide stimulation. Release of prostaglandin E1 and leukotriene B4 were significantly decreased in group A, whereas prostaglandin E2 and interleukin-10 concentrations were significantly increased in group B. No effect on interleukin-8 or tumor necrosis factor-α release was found after supplementation with either fat blend. Conclusions These results show an immune-modulating effect of a low-dose dietary polyunsaturated fatty acid supplementation. However, further studies regarding fat-blend composition and period of supplementation in patients with inflammatory conditions are required.