Comparison of the effects of desflurane and sevoflurane on the expression of platelet surface glycoproteins in unstimulated and adenosine diphosphate-induced platelets in vitro

• Published in: Journal of clinical anesthesia 2007-08, Vol.19 (5), p.328-333
• Main Authors: Koroglu, Ahmet, MD, Cicek, Muslum, MD, Toprak, Huseyin I., MD, Karakoc, Yunus, MD, Noyan, Funda, MD, Ersoy, Ozcan M., MD
• Format: Article
• Language: English
• Subjects: Adenosine; Adenosine Diphosphate - pharmacology; Adult; Anesthesia; Anesthesia & Perioperative Care; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Anesthetics, Inhalation - pharmacology; Biological and medical sciences; Blood platelets; Blood Platelets - drug effects; Blood Platelets - metabolism; Cardiovascular disease; Cell culture; Chlorophyll; Cloning; Desflurane; Flow Cytometry - methods; Humans; Isoflurane - analogs & derivatives; Isoflurane - pharmacology; Medical sciences; Methyl Ethers - pharmacology; P-Selectin - blood; Pain Medicine; Platelet; Platelet Activation - drug effects; Platelet Membrane Glycoproteins - metabolism; Prospective Studies; Sevoflurane; Studies; Surface glycoproteins; Volatile anesthetic
• ISSN: 0952-8180; 1873-4529
• DOI: 10.1016/j.jclinane.2007.02.002

Abstract Study Objective To compare the effects of one minimum alveolar concentration (MAC) desflurane and sevoflurane on the expression of CD42b (glycoprotein [GP] Ib), CD41 (GPIIb), CD61 (GPIIIa), CD62P (P-selectin), and CD63 in both unstimulated and adenosine diphosphate (ADP)–stimulated platelets in vitro. Setting University laboratory. Subjects 15 healthy volunteers. Interventions Platelet-rich plasma was obtained and divided into three groups: platelet-rich plasma exposed to air (group 1); air plus one MAC desflurane (6% vol; group 2), and air plus one MAC sevoflurane (2% vol; group 3), for 40 minutes. Percentage of antigen-positive cells (%+ ) mean channel fluorescence (MCFΣ ), and index of platelet activation for positive platelets (IPA+ ) as expression markers for GPIb, GPIIb, GPIIIa, P-selectin, and CD63, were measured. Measurements and Main Results In unstimulated platelets, expression markers for GPIIb and GPIIIa were significantly lower in groups 2 and 3 than group 1 ( P < 0.001). P-selectin expression markers were significantly higher in group 2 than in group 1 or group 3 ( P < 0.016). CD63 expression markers were significantly lower in group 3 than group 1 ( P < 0.016). In ADP-stimulated platelets, expression markers for all glycoproteins were significantly higher in all groups. Conclusion Neither one MAC desflurane nor sevoflurane showed any significant change in ADP-stimulated platelets compared with the control group.