Differential Effects of Two Period Genes on the Physiology and Proteomic Profiles of Mouse Anterior Tibialis Muscles

• Published in: Molecules and cells 2006-12, Vol.22 (3), p.275-284
• Main Authors: Bae, K.H. (Yonsei University, Wonju, Republic of Korea), E-mail: [e-mail], Lee, K.S. (Yonsei University, Wonju, Republic of Korea), Seo, Y.U. (Yonsei University, Wonju, Republic of Korea), Lee, H.S. (Yonsei University, Wonju, Republic of Korea), Kim, D.Y. (Yonsei University, Wonju, Republic of Korea), Choi, I.H. (Yonsei University, Wonju, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: Animals; Biological Clocks; BIOLOGICAL RHYTHMS; Blood Proteins - metabolism; Cell Cycle Proteins - genetics; Circadian Clock; Circadian Rhythm; Contractile Proteins - metabolism; Electrophoresis, Gel, Two-Dimensional; Heat-Shock Proteins - metabolism; Immunoblotting; Mice; Mice, Inbred Strains; Mice, Knockout; Motor Activity; mPer Mutant Mice; Muscle Contraction; Muscle Proteins - metabolism; Muscle, Skeletal - anatomy & histology; Muscle, Skeletal - chemistry; Muscle, Skeletal - physiology; Nuclear Proteins - genetics; Period Circadian Proteins; Proteomics; RITMOS BIOLOGICOS; RYTHME BIOLOGIQUE; Transcription Factors - genetics; Treadmill Running; Two-dimensional Gel Electrophoresis
• ISSN: 1016-8478
• DOI: 10.1016/S1016-8478(23)17421-8

The molecular components that generate and maintain circadian rhythms of physiology and behavior in mammals are present both in the brain (suprachiasmatic nucleus; SCN) and in peripheral tissues. Examination of mice with targeted disruptions of either mPer1 or mPer2 has shown that these two genes have key roles in the SCN circadian clock. Here we show that loss of the clock gene mPer2 affects forced locomotor performance in mice without altering muscle contractility. A proteomic analysis revealed that the anterior tibialis muscles of the mPer2 knockout mice had higher levels of glycolytic enzymes such as triose phosphate isomerase and enolase than those of either the wild type or mPer1 knockout mice.