Fluid resuscitation with hemoglobin-vesicle solution does not increase hypoxia or inflammatory responses in moderate hemorrhagic shock

The aim of the present study was to compare the hypoxic and inflammatory effects of transfusing hemoglobin-vesicles (HbV) or lactated Ringer's (LR) solution on several organs in a hemorrhagic shock model. Hemorrhagic shock was induced in 48 anesthetized rats by withdrawing 28 mL/kg blood. The a...

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Bibliographic Details
Published in:Biomedical Research 2006, Vol.27(6), pp.283-288
Main Authors: GOTO, Yoshitugu, TERAJIMA, Katsuyuki, TSUESHITA, Takaya, MIYASHITA, Masao, HORINOUCHI, Hirohisa, SAKAI, Hiromi, TSUCHIDA, Eishun, SAKAMOTO, Atsuhiro
Format: Article
Language:English
Subjects:
Animals
Blood Pressure
Blood Substitutes - adverse effects
Hemoglobins - adverse effects
Hypoxia - chemically induced
Inflammation - chemically induced
Isotonic Solutions - adverse effects
Lactic Acid - blood
Male
Organ Specificity
Rats
Rats, Sprague-Dawley
Resuscitation
RNA, Messenger - metabolism
Shock, Hemorrhagic - drug therapy
Tumor Necrosis Factor-alpha - metabolism
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Summary:The aim of the present study was to compare the hypoxic and inflammatory effects of transfusing hemoglobin-vesicles (HbV) or lactated Ringer's (LR) solution on several organs in a hemorrhagic shock model. Hemorrhagic shock was induced in 48 anesthetized rats by withdrawing 28 mL/kg blood. The animals were resuscitated by replacing the blood with an equal volume of HbV solution or three times the volume of LR solution. The heart, lung, liver, kidney and spleen were extracted at different time points following resuscitation, and mRNA expression levels of hypoxia-induced factor 1-alpha (HIF-1α) and tumor necrosis factor-alpha (TNF-α) were determined. Blood lactate concentrations in the HbV group rapidly returned to baseline levels, whereas elevated lactate concentrations in the LR group were prolonged. There were no significant differences between the two resuscitation groups in terms of HIF-1α and TNF-α expression in the organs examined. HIF-1α and TNF-α expression in the lungs was significantly greater than in other organs. Our results suggest that resuscitation from hemorrhagic shock with HbV did not increase hypoxic or inflammatory effects in major organs, compared with resuscitation using LR solution, despite prolonged elevation of blood lactate.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.27.283