Digital image processing as a tool to monitor biomass growth in Aspergillus niger 3T5B8 solid-state fermentation: preliminary results

The estimation of biomass is an essential parameter for controlling fermentation processes. However, monitoring biomass growth in filamentous fungi solid-state fermentation is laborious. The aim of this study was to provide a better insight into the monitoring of biomass growth in Aspergillus niger...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2006-12, Vol.224 (3), p.290-297
Main Authors: COURI, S, MERCÊS, E.P, NEVES, B.C.V, SENNA, L.F
Format: Article
Language:English
Subjects:
Aspergillus niger
Aspergillus niger - growth & development
Biomass
Biomass growth
Biotechnology - methods
Culture Media
digital image processing
Fermentation
filamentous fungi
glycosamine analysis
Monitoring, Physiologic - methods
polygalacturonase
solid‐state fermentation
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Summary:The estimation of biomass is an essential parameter for controlling fermentation processes. However, monitoring biomass growth in filamentous fungi solid-state fermentation is laborious. The aim of this study was to provide a better insight into the monitoring of biomass growth in Aspergillus niger 3T5B8 solid-state fermentation using a digital image-processing technique. The images were acquired with a stereomicroscope and a digital camera, and processed using KS400 software. Growth was evaluated every 24 h for 5 days, and quantified as the total area occupied by the hyphae. The correlation between the results of the proposed methodology and the polygalacturonase data was greater than 0.9, showing that a direct and linear relationship can be expected among these parameters. This work indicates that the digital processing technique can be used for indirect biomass estimation in a solid-state fermentation process.
ISSN:0022-2720
1365-2818
DOI:10.1111/j.1365-2818.2006.01699.x