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Ultrasound-Enhanced Chemotherapy and Gene Delivery for Glioma Cells

Treatment of brain cancer is limited in part by inefficient intracellular delivery of drugs and DNA for chemotherapy and gene therapy, respectively. This study tested the hypothesis that ultrasound may be used to enhance intracellular delivery and efficacy of chemotherapeutics and genes in glioma ce...

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Published in:Technology in cancer research & treatment 2007-10, Vol.6 (5), p.433-442
Main Authors: Zarnitsyn, Vladimir G., Kamaev, Pavel P., Prausnitz, Mark R.
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container_title Technology in cancer research & treatment
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creator Zarnitsyn, Vladimir G.
Kamaev, Pavel P.
Prausnitz, Mark R.
description Treatment of brain cancer is limited in part by inefficient intracellular delivery of drugs and DNA for chemotherapy and gene therapy, respectively. This study tested the hypothesis that ultrasound may be used to enhance intracellular delivery and efficacy of chemotherapeutics and genes in glioma cells in vitro. First, suitable ultrasound conditions were identified by measuring intracellular uptake of calcein and viability of GS 9L rat gliosarcoma cells after a range of different ultrasound exposures. We selected sonication at 10 J/cm2, which achieved intracellular delivery of ν106 molecules/cell. Next, glial cells were sonicated with varying concentrations of model chemotherapeutics: BCNU and bleomycin. For both drugs, cytotoxicity was increased in a synergistic manner when accompanied by ultrasound exposure. Finally, expression of a plasmid DNA encoding a GFP reporter was increased up to 30-fold when exposed to ultrasound. Altogether, these findings suggest that ultrasound may be useful to increase the efficacy of chemotherapy and gene therapy of glioma cells.
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This study tested the hypothesis that ultrasound may be used to enhance intracellular delivery and efficacy of chemotherapeutics and genes in glioma cells in vitro. First, suitable ultrasound conditions were identified by measuring intracellular uptake of calcein and viability of GS 9L rat gliosarcoma cells after a range of different ultrasound exposures. We selected sonication at 10 J/cm2, which achieved intracellular delivery of ν106 molecules/cell. Next, glial cells were sonicated with varying concentrations of model chemotherapeutics: BCNU and bleomycin. For both drugs, cytotoxicity was increased in a synergistic manner when accompanied by ultrasound exposure. Finally, expression of a plasmid DNA encoding a GFP reporter was increased up to 30-fold when exposed to ultrasound. 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Phacomatoses</topic><topic>Ultrasonic Therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zarnitsyn, Vladimir G.</creatorcontrib><creatorcontrib>Kamaev, Pavel P.</creatorcontrib><creatorcontrib>Prausnitz, Mark R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Technology in cancer research &amp; treatment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Zarnitsyn, Vladimir G.</au><au>Kamaev, Pavel P.</au><au>Prausnitz, Mark R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasound-Enhanced Chemotherapy and Gene Delivery for Glioma Cells</atitle><jtitle>Technology in cancer research &amp; treatment</jtitle><addtitle>Technol Cancer Res Treat</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>6</volume><issue>5</issue><spage>433</spage><epage>442</epage><pages>433-442</pages><issn>1533-0346</issn><eissn>1533-0338</eissn><coden>TCRTBS</coden><abstract>Treatment of brain cancer is limited in part by inefficient intracellular delivery of drugs and DNA for chemotherapy and gene therapy, respectively. 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source SAGE Open Access Journals
subjects Animals
Antineoplastic Agents - administration & dosage
Biological and medical sciences
Bleomycin - administration & dosage
Carmustine - administration & dosage
Cell Survival
Combined Modality Therapy
DNA - administration & dosage
Dose-Response Relationship, Drug
Gene Transfer Techniques
Genetic Therapy - methods
Gliosarcoma - therapy
Green Fluorescent Proteins - administration & dosage
Green Fluorescent Proteins - genetics
Medical sciences
Neurology
Rats
Transfection
Tumor Cells, Cultured
Tumors of the nervous system. Phacomatoses
Ultrasonic Therapy
title Ultrasound-Enhanced Chemotherapy and Gene Delivery for Glioma Cells
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