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Immunohistochemical detection of galectin-1 in renal biopsy specimens of children and its possible role in proteinuric glomerulopathies

Aims:  Galectin‐1 is an endogenous lectin that specifically binds to β‐galactoside structures. It has been associated with developmental mechanisms ranging from differentiation to apoptosis and exerts immunoregulatory functions in autoimmune diseases. The aim was to determine the immunohistochemical...

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Published in:Histopathology 2007-10, Vol.51 (4), p.468-476
Main Authors: Ostalska-Nowicka, D, Zachwieja, J, Nowicki, M, Kaczmarek, E, Siwińska, A, Witt, M
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container_title Histopathology
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creator Ostalska-Nowicka, D
Zachwieja, J
Nowicki, M
Kaczmarek, E
Siwińska, A
Witt, M
description Aims:  Galectin‐1 is an endogenous lectin that specifically binds to β‐galactoside structures. It has been associated with developmental mechanisms ranging from differentiation to apoptosis and exerts immunoregulatory functions in autoimmune diseases. The aim was to determine the immunohistochemical expression of galectin‐1 in renal biopsy specimens of children with primary idiopathic proteinuric glomerulopathies. Methods and results:  We examined 18 children with minimal change disease (MCD), 30 with diffuse mesangial proliferation (DMP) and 11 with focal segmental glomerulosclerosis (FSGS). An indirect immunohistochemical protocol using a polyclonal antibody directed against galectin‐1 was applied. Galectin‐1 was detected in renal podocytes in DMP and FSGS cases, while control glomeruli and MCD were negative. Galectin‐1 immunoreactivity was found within parietal epithelial cells in patients with FSGS. Conclusions:  These results suggest a possible role for galectin‐1 in the pathogenesis of primary glomerulopathies in children as a kind of podocyte‐related self‐protective activity and probably involvement of epithelial cells of Bowman’s capsule in inflammatory processes. Immunohistochemistry using galectin‐1 antibodies may further be helpful in histological distinction between MCD and DMP.
doi_str_mv 10.1111/j.1365-2559.2007.02818.x
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It has been associated with developmental mechanisms ranging from differentiation to apoptosis and exerts immunoregulatory functions in autoimmune diseases. The aim was to determine the immunohistochemical expression of galectin‐1 in renal biopsy specimens of children with primary idiopathic proteinuric glomerulopathies. Methods and results:  We examined 18 children with minimal change disease (MCD), 30 with diffuse mesangial proliferation (DMP) and 11 with focal segmental glomerulosclerosis (FSGS). An indirect immunohistochemical protocol using a polyclonal antibody directed against galectin‐1 was applied. Galectin‐1 was detected in renal podocytes in DMP and FSGS cases, while control glomeruli and MCD were negative. Galectin‐1 immunoreactivity was found within parietal epithelial cells in patients with FSGS. Conclusions:  These results suggest a possible role for galectin‐1 in the pathogenesis of primary glomerulopathies in children as a kind of podocyte‐related self‐protective activity and probably involvement of epithelial cells of Bowman’s capsule in inflammatory processes. Immunohistochemistry using galectin‐1 antibodies may further be helpful in histological distinction between MCD and DMP.</description><identifier>ISSN: 0309-0167</identifier><identifier>EISSN: 1365-2559</identifier><identifier>DOI: 10.1111/j.1365-2559.2007.02818.x</identifier><identifier>PMID: 17880528</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Biological and medical sciences ; Biopsy ; Child ; Child, Preschool ; Diagnosis, Differential ; Female ; Galectin 1 - metabolism ; galectin-1 ; Glomerulosclerosis, Focal Segmental - diagnosis ; Glomerulosclerosis, Focal Segmental - metabolism ; Humans ; immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; kidney ; Kidney - metabolism ; Kidney - pathology ; Male ; Medical sciences ; Nephrology. Urinary tract diseases ; Nephrosis, Lipoid - diagnosis ; Nephrosis, Lipoid - metabolism ; nephrotic syndrome ; Nephrotic Syndrome - diagnosis ; Nephrotic Syndrome - etiology ; Nephrotic Syndrome - metabolism ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; podocytes ; Podocytes - metabolism ; Podocytes - pathology ; Urinary system involvement in other diseases. Miscellaneous ; Urinary tract. 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It has been associated with developmental mechanisms ranging from differentiation to apoptosis and exerts immunoregulatory functions in autoimmune diseases. The aim was to determine the immunohistochemical expression of galectin‐1 in renal biopsy specimens of children with primary idiopathic proteinuric glomerulopathies. Methods and results:  We examined 18 children with minimal change disease (MCD), 30 with diffuse mesangial proliferation (DMP) and 11 with focal segmental glomerulosclerosis (FSGS). An indirect immunohistochemical protocol using a polyclonal antibody directed against galectin‐1 was applied. Galectin‐1 was detected in renal podocytes in DMP and FSGS cases, while control glomeruli and MCD were negative. Galectin‐1 immunoreactivity was found within parietal epithelial cells in patients with FSGS. Conclusions:  These results suggest a possible role for galectin‐1 in the pathogenesis of primary glomerulopathies in children as a kind of podocyte‐related self‐protective activity and probably involvement of epithelial cells of Bowman’s capsule in inflammatory processes. Immunohistochemistry using galectin‐1 antibodies may further be helpful in histological distinction between MCD and DMP.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Diagnosis, Differential</subject><subject>Female</subject><subject>Galectin 1 - metabolism</subject><subject>galectin-1</subject><subject>Glomerulosclerosis, Focal Segmental - diagnosis</subject><subject>Glomerulosclerosis, Focal Segmental - metabolism</subject><subject>Humans</subject><subject>immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>kidney</subject><subject>Kidney - metabolism</subject><subject>Kidney - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nephrosis, Lipoid - diagnosis</subject><subject>Nephrosis, Lipoid - metabolism</subject><subject>nephrotic syndrome</subject><subject>Nephrotic Syndrome - diagnosis</subject><subject>Nephrotic Syndrome - etiology</subject><subject>Nephrotic Syndrome - metabolism</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>podocytes</subject><subject>Podocytes - metabolism</subject><subject>Podocytes - pathology</subject><subject>Urinary system involvement in other diseases. Miscellaneous</subject><subject>Urinary tract. 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Conclusions:  These results suggest a possible role for galectin‐1 in the pathogenesis of primary glomerulopathies in children as a kind of podocyte‐related self‐protective activity and probably involvement of epithelial cells of Bowman’s capsule in inflammatory processes. Immunohistochemistry using galectin‐1 antibodies may further be helpful in histological distinction between MCD and DMP.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17880528</pmid><doi>10.1111/j.1365-2559.2007.02818.x</doi><tpages>9</tpages></addata></record>
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source Wiley-Blackwell Read & Publish Collection
subjects Adolescent
Biological and medical sciences
Biopsy
Child
Child, Preschool
Diagnosis, Differential
Female
Galectin 1 - metabolism
galectin-1
Glomerulosclerosis, Focal Segmental - diagnosis
Glomerulosclerosis, Focal Segmental - metabolism
Humans
immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
kidney
Kidney - metabolism
Kidney - pathology
Male
Medical sciences
Nephrology. Urinary tract diseases
Nephrosis, Lipoid - diagnosis
Nephrosis, Lipoid - metabolism
nephrotic syndrome
Nephrotic Syndrome - diagnosis
Nephrotic Syndrome - etiology
Nephrotic Syndrome - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
podocytes
Podocytes - metabolism
Podocytes - pathology
Urinary system involvement in other diseases. Miscellaneous
Urinary tract. Prostate gland
title Immunohistochemical detection of galectin-1 in renal biopsy specimens of children and its possible role in proteinuric glomerulopathies
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