Prevalence of human papillomavirus genotypes in women with normal and abnormal cervical cytology in Iran

HPV infection has a prime etiologic role in development and progression of cervical cancer, one of the most frequent forms of cancer among women in developing countries. This study was designed to determine the most prevalent HPV genotypes in women with normal and abnormal cervical cytology in Iran....

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Bibliographic Details
Published in:Asian Pacific journal of cancer prevention : APJCP 2006-10, Vol.7 (4), p.529-532
Main Authors: Ghaffari, Saeed Reza, Sabokbar, Tayebeh, Mollahajian, Hamid, Dastan, Jila, Ramezanzadeh, Fateme, Ensani, Fereshteh, Yarandi, Fariba, Mousavi-Jarrahi, Alireza, Mohagheghi, Mohammad Ali, Moradi, Abdolvahab
Format: Article
Language:English
Subjects:
Adult
Cervix Uteri - virology
Chi-Square Distribution
DNA, Viral - analysis
Female
Genotype
Humans
Iran - epidemiology
Papillomaviridae - classification
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - epidemiology
Papillomavirus Infections - genetics
Polymerase Chain Reaction
Prevalence
Risk Factors
Statistics, Nonparametric
Uterine Cervical Diseases - epidemiology
Uterine Cervical Diseases - genetics
Uterine Cervical Diseases - virology
Uterine Cervical Neoplasms - epidemiology
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - virology
Vaginal Smears
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Summary:HPV infection has a prime etiologic role in development and progression of cervical cancer, one of the most frequent forms of cancer among women in developing countries. This study was designed to determine the most prevalent HPV genotypes in women with normal and abnormal cervical cytology in Iran. Samples from134 patients, including 127 who attended gynecology clinics and 7 with solid cervical tumors were used. All 127 patients underwent routine Pap tests for cytological evaluation and at the same visit a sample of cervical epithelial cells was obtained by scraping the cervix osteum. In each case HPV infection was primarily evaluated by PCR using GP 5/6 primers and then subtyping was performed in proved infected samples with specific primers for HPV 16, 18, 31, 33, 11 and 6. After cytological evaluation, 50 patients with abnormal Pap tests were categorized as the abnormal group and the remaining 77 patients as the normal group. In the normal group, HPV infection was established in 10 cases (13% infection rate), while 30 HPV positive cases were discovered in the abnormal group (60% infected). The most prevalent genotypes among the infected samples were HPV 16 (76%), HPV18 (12.7%) and HPV11/6 (8.5%). Moreover, all 7 tumor samples were positive for HPV general primers of which, 5 samples were infected with HPV 16, two were co-infected with HPV16,18 and HPV16,31 genotypes and one was infected with HPV 18. Infection with HPV 16 was found to be significantly higher in abnormal group in comparison with normal group (42% vs. 11.6%, P value
ISSN:1513-7368