The structure of the carbohydrate backbone of the LPS from Myxococcus xanthus strain DK1622

where ac are 13-methyl-C14-3OH ( iso-C15-3OH), C16-3OH, or 15-methyl-C16-3OH ( iso-C17-3OH). Gram-negative rod shaped bacterium Myxococcus xanthus DK1622 produces a smooth-type LPS. The structure of the polysaccharide O-chain and the core-lipid A region of the LPS has been determined by chemical and...

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Bibliographic Details
Published in:Carbohydrate research 2007-11, Vol.342 (16), p.2474-2480
Main Authors: MacLean, Leann, Perry, Malcolm B., Nossova, Ludmila, Kaplan, Heidi, Vinogradov, Evgeny
Format: Article
Language:English
Subjects:
Carbohydrate Sequence
Core
Lipopolysaccharides - chemistry
LPS
Magnetic Resonance Spectroscopy
Myxococcus xanthus - chemistry
Myxococcus xanthus - classification
NMR
Polysaccharide
Spectrometry, Mass, Electrospray Ionization
Structure
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Summary:where ac are 13-methyl-C14-3OH ( iso-C15-3OH), C16-3OH, or 15-methyl-C16-3OH ( iso-C17-3OH). Gram-negative rod shaped bacterium Myxococcus xanthus DK1622 produces a smooth-type LPS. The structure of the polysaccharide O-chain and the core-lipid A region of the LPS has been determined by chemical and spectroscopic methods. The O-chain was built up of disaccharide repeating units having the following structure: → 6 ) - α - d-Glcp- ( 1 → 4 ) - α - d-GalpNAc 6 OMe ∗ - ( 1 → with partially methylated GalNAc residue. The core region consisted of a phosphorylated hexasaccharide, containing one Kdo residue, unsubstituted at O-4, and no heptose residues. The lipid A component consisted of β-GlcN-(1→6)-α-GlcN1P disaccharide, N-acylated with 13-methyl-C14-3OH ( iso-C15-3OH), C16-3OH, and 15-methyl-C16-3OH ( iso-C17-3OH) acids. The lipid portion contained O-linked iso-C16 acid.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2007.07.023