Cloning and sequence analysis of ornithine decarboxylase gene fragments from the Ascomycota

Ornithine decarboxylase (ODC; EC 4.1.1.17) catalyzes the initial step in the biosynthesis of polyamines, the conversion of ornithine to putrescine. Based on the most conserved regions of fungal ODCs, we designed and synthesized oligonucleotides to amplify homologous fragments of three important plan...

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Bibliographic Details
Published in:DNA sequence 2006-06, Vol.17 (3), p.231-236
Main Authors: Jiménez-Bremont, Juan Francisco, Rodríguez-Kessler, Margarita, Rodríguez-Guerra, Raul, Cortes-Penagos, Carlos, Torres-Guzman, Juan Carlos, Williamson, June Simpson
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Ascomycota
Ascomycota - enzymology
Ascomycota - genetics
Base Sequence
Binding Sites
Cloning, Molecular
Colletotrichum lindemuthianum
Conserved Sequence
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fusarium graminearum
Fusarium solani
Magnaporthe grisea
Metarhizium anisopliae
Molecular Sequence Data
Neurospora crassa
Ornithine Decarboxylase - chemistry
Ornithine Decarboxylase - genetics
Phylogeny
Sequence Alignment
Sequence Analysis
Sequence Homology
Sordariales
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Summary:Ornithine decarboxylase (ODC; EC 4.1.1.17) catalyzes the initial step in the biosynthesis of polyamines, the conversion of ornithine to putrescine. Based on the most conserved regions of fungal ODCs, we designed and synthesized oligonucleotides to amplify homologous fragments of three important plant pathogenic Pyrenomycete fungi (Ascomycota), Magnaporthe grisea, Colletotrichum lindemuthianum and Fusarium solani, and one insect pathogenic fungus Metarhizium anisopliae. Cloning and sequencing of the amplified fragments revealed homologies of between 37 to 88% with other fungal ODCs. The predicted peptide sequences were compared by Clustal analysis and conserved sequences corresponding to the substrate and cofactor binding sites were identified. Comparative analyses of the ODC fragments isolated in this study, revealed high homology between them (68.3-81.1%) and also with other Pyrenomycetes such as Neurospora crassa (order Sordariales; 68.6-72.9%) and Fusarium graminearum (order Hypocreales; 70.8-88.1%). Data obtained in this work revealed that these fungi constitute a compact group separated from other eukaryotic ODCs.
ISSN:1042-5179
1029-2365
DOI:10.1080/10425170600807009