An accurate and easy procedure to obtain isothermal Kováts retention indices in gas chromatography

• Published in: Journal of separation science 2006-12, Vol.29 (18), p.2785-2792
• Main Authors: García-Domínguez, José Antonio, Lebrón-Aguilar, Rosa, Quintanilla-López, Jesús Eduardo
• Format: Article
• Language: English
• Subjects: Accuracy; Alkanes; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Gas chromatographic methods; Gas chromatography; Retention indices
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200600110

Isothermal Kováts retention indices may be used in GC for identification purposes, but they are also useful in characterisation of stationary phases and for studying structural and physico‐chemical properties of both the analyte and the stationary phase. They are currently reported as whole numbers with an accuracy of one index unit for non‐polar stationary phases. The method recommended for their calculation uses a linear regression model, although non‐linear models have also been applied with good results. In both cases, a computer programme and the retention times of a series of n‐alkanes that elute correctly and resolved from the other compounds are needed, conditions which cannot always be fulfilled. However, it is possible to calculate retention indices with an accuracy of 0.1 retention index units (0.2 units for packed columns) with the help of only three n‐alkanes using just a pocket calculator or a computer spreadsheet. The main requirement is that at least one of the n‐alkanes has a retention index differing by no more than one hundred retention index units from that of the analyte being investigated. Examples are given for different stationary phases.