Insights in the Organization of DNA−Surfactant Monolayers Using Cryo-Electron Tomography

We demonstrate that for the present system the general view on DNA−cationic surfactant Langmuir monolayers, in which DNA is bound directly underneath a closed monolayer surface, is only applicable when DNA is injected underneath a preformed monolayer. Moreover, cryo-electron tomography showed that t...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2007-10, Vol.129 (39), p.11894-11895
Main Authors: Vos, Matthijn R. J, Bomans, Paul H. H, de Haas, Felix, Frederik, Peter M, Jansen, John A, Nolte, Roeland J. M, Sommerdijk, Nico A. J. M
Format: Article
Language:English
Subjects:
Cryoelectron Microscopy - methods
DNA - chemistry
Nucleic Acid Conformation
Surface-Active Agents - chemistry
Urea - analogs & derivatives
Urea - chemistry
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Summary:We demonstrate that for the present system the general view on DNA−cationic surfactant Langmuir monolayers, in which DNA is bound directly underneath a closed monolayer surface, is only applicable when DNA is injected underneath a preformed monolayer. Moreover, cryo-electron tomography showed that the DNA strands do not bind completely to the monolayer surface in an orderly fashion, but partially extend down into the subphase.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0736515