Expression of P-glycoprotein in lymphocytes of children with nephrotic syndrome treated with glucocorticoids

Glucocorticoids are still the mainstay of therapy for nephrotic syndrome (NS) in children. Poor response to glucocorticoids may relate, in part, to the overexpression of P-glycoprotein (P-gp). The aim of the present study was to determine the expression of P-gp in lymphocytes (CD3) in the peripheral...

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Published in:European journal of pediatrics 2006-12, Vol.165 (12), p.839-844
Main Authors: WASILEWSKA, Anna M, ZOCH-ZWIERZ, Walentyna M, PIETRUCZUK, Miroslawa
Format: Article
Language:English
Subjects:
Adolescent
ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism
Biological and medical sciences
Case-Control Studies
CD3 Complex
Child
Child, Preschool
Cytokines
Dose-Response Relationship, Drug
Drug dosages
Female
Flow Cytometry
General aspects
Glomerulonephritis
Glucocorticoids - pharmacology
Glucocorticoids - therapeutic use
Glycoproteins
Hormones
Humans
Kidney diseases
Lymphocyte Count
Lymphocytes
Male
Medical sciences
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Nephrotic Syndrome - drug therapy
Nephrotic Syndrome - metabolism
Prednisone - pharmacology
Prednisone - therapeutic use
Recurrence
Remission (Medicine)
Remission Induction
Steroids
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
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Summary:Glucocorticoids are still the mainstay of therapy for nephrotic syndrome (NS) in children. Poor response to glucocorticoids may relate, in part, to the overexpression of P-glycoprotein (P-gp). The aim of the present study was to determine the expression of P-gp in lymphocytes (CD3) in the peripheral blood of children with steroid-sensitive nephrotic syndrome in the dynamics of the disease. The study group (I) consisted of 18 children, median age 5.75 years, with steroid-sensitive nephrotic syndrome, in whom the examinations were carried out three times: (A) before treatment, during relapse; (B) after 3-4 weeks of prednisone treatment; (C) 2 months after finishing prednisone treatment. The control group (II) consisted of 18 healthy children of the same age. P-gp expression in CD3 lymphocytes of peripheral blood was measured using flow cytometry. During NS relapse and prior to glucocorticoid administration, the CD3/P-gp level was higher (median 3.20%, range 0.80-7.80%) when compared to healthy controls (1.10%, range 0.30- 2.20%) (p
ISSN:0340-6199
1432-1076
DOI:10.1007/s00431-006-0177-1