Cytokine expression in naïve and previously infected lambs after challenge with Teladorsagia circumcincta

• Published in: Veterinary immunology and immunopathology 2007-11, Vol.120 (1), p.47-54
• Main Authors: Craig, N.M., Miller, H.R.P., Smith, W.D., Knight, P.A.
• Format: Article
• Language: English
• Subjects: abomasum; Animals; biological resistance; Cytokine; cytokines; Cytokines - genetics; Cytokines - metabolism; disease resistance; gastrointestinal nematodes; gene expression; Gene Expression Regulation - immunology; Helminth; immune response; immune system; immunization; immunologic factors; interleukins; lymph nodes; Nematoda; nematode infections; normal values; protective effect; RT-PCR; Sheep; sheep diseases; Sheep Diseases - immunology; Sheep Diseases - metabolism; Sheep Diseases - parasitology; Teladorsagia circumcincta; transcriptional activation; transforming growth factors; Trichostrongyloidea - physiology; Trichostrongyloidiasis - immunology; Trichostrongyloidiasis - metabolism; Trichostrongyloidiasis - veterinary; tumor necrosis factor-alpha
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2007.07.004

Infection of sheep with Teladorsagia circumcincta triggers an immune response with predominantly type-2 (Th2) characteristics, including local eosinophila, mastocytosis and increased mucus production. In order to better understand the protective immune responses elicited, we used RT-PCR assays to define the changes in expression levels of a range of cytokine transcripts in lymph nodes draining the ovine abomasum following a challenge infection with T. circumcincta. This study compared the changes in cytokine expression in the abomasal lymph node following challenge with T. circumcincta in naïve sheep (Group 2) and sheep immunised by a previous trickle infection (Group 3), in comparison to unchallenged naive sheep (Group 1). There was a significant up-regulation of interleukin-4 (IL-4), IL-5 and IL-13 in both the challenged groups compared to naïve individuals. There was also an up-regulation of IL-1β, IL-6, IL-10, IL-18, transforming growth factor-β 1 (TGFβ 1) and tumour necrosis factor-α (TNFα) by day 5 after infection. IL-12p 40 was found to be increased in the previously infected Group 3 animals by day 5 following challenge. By contrast, transcription of this cytokine was found to be reduced by day 10 following infection of Group 2 animals. Expression of IL-2 and Interferon-γ (IFNγ) did not significantly differ between the three groups.