MAPKinase Gene Expression, as Determined by Microarray Analysis, Distinguishes Uncomplicated from Complicated Reconstitution after Major Surgical Trauma

• Published in: Annals of the New York Academy of Sciences 2006-12, Vol.1090 (1), p.429-444
• Main Authors: SCHNEIDER, E. MARION, WEISS, MANFRED, DU, WEIDONG, LEDER, GERHARD, BUTTENSCHÖN, KLAUS, LIENER, ULRICH C., BRÜCKNER, UWE B.
• Format: Article
• Language: English
• Subjects: Adult; Aged, 80 and over; cytokines; Female; Gene Expression Regulation, Enzymologic; hematopoiesis; Humans; MAPkinase; microarray; Middle Aged; Mitogen-Activated Protein Kinases - metabolism; Oligonucleotide Array Sequence Analysis; outcome; reconstitution; RNA, Messenger - genetics; sepsis; sepsis, MAPkinase; Surgical Procedures, Operative - adverse effects; tissue regeneration; transcription factors; Transcription Factors - metabolism; trauma-induced gene expression; Wounds and Injuries - enzymology; Wounds and Injuries - genetics; Wounds and Injuries - metabolism; Wounds and Injuries - physiopathology
• ISSN: 0077-8923; 1749-6632; 1930-6547
• DOI: 10.1196/annals.1378.046

:  Microarray expression analysis was performed in patients with major surgical trauma to identify signaling pathways which may be indicative for complicated versus uneventful reconstitution post trauma. In addition to a generalized upregulation of nonspecific stress response genes in all patients, a remarkable number of differences in gene expression patterns were found in individual patients. Some of the differing genes were associated with uncomplicated convalescence such as upregulation of both the ERK5 pathway (MAPK7 [mitogen‐activated protein kinase‐7]) and transcription factors which stimulate hematopoiesis and tissue reconstitution (MEF2, BMP‐2, TNFRSF11A [RANK], and RUNX‐1). Chemokine genes active in stem cell recruitment from the bone marrow as well as dendritic cell and natural killer (NK) cell maturation (SCYA14 [HCC‐1]), and activators of the lymphoid compartment (TNFRSF7 [CD27], CD3zeta and perforin [PRF1]) were increased. In contrast, all these transcripts were downregulated in complicated reconstitution and later development of septic shock. Moreover, p38 kinase (MAPK14), S100 molecules, and members of the lipoxygenase pathway were associated with a more eventful outcome. Microarray expression studies are a promising tool for screening and then selecting differentially regulated genes in favorable as compared to complicated reconstitution post trauma.