Expression of Toll-like receptor 4 in the basilar artery after experimental subarachnoid hemorrhage in rabbits: A preliminary study

Abstract Inflammation and immunity play a crucial role in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage (SAH). Recently, a growing body of evidence indicates that Toll-like receptor (TLR) 4 is vital for inflammation and immunity. Therefore, this study aimed to detect the expre...

Full description

Saved in:
Bibliographic Details
Published in:Brain research 2007-10, Vol.1173, p.110-116
Main Authors: Zhou, Meng-liang, Wu, Wei, Ding, Ya-suo, Zhang, Fei-fan, Hang, Chun-hua, Wang, Han-dong, Cheng, Hui-lin, Yin, Hong-xia, Shi, Ji-xin
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Basilar Artery - metabolism
Biological and medical sciences
Cerebral vasospasm
Disease Models, Animal
Gene Expression Regulation - physiology
Inflammation
Male
Medical sciences
Neurology
Rabbits
Subarachnoid hemorrhage
Subarachnoid Hemorrhage - pathology
Time Factors
Toll-like receptor 4
Toll-Like Receptor 4 - metabolism
Vascular diseases and vascular malformations of the nervous system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Inflammation and immunity play a crucial role in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage (SAH). Recently, a growing body of evidence indicates that Toll-like receptor (TLR) 4 is vital for inflammation and immunity. Therefore, this study aimed to detect the expression of TLR4 in the basilar artery in a rabbit SAH model and to clarify the potential role of TLR4 in cerebral vasospasm. A total of 48 rabbits were randomly divided into four groups: control group; day 3, day 5, and day 7 groups. Day 3, day 5, and day 7 groups were all SAH groups. The animals in day 3, day 5 and day 7 groups were subjected to injection of autologous blood into cisterna magna twice on day 0 and day 2 and were killed on days 3, 5, and 7, respectively. Cross-sectional area of basilar artery was measured and the TLR4 expression was assessed by immunohistochemistry and Western blot analysis. The basilar arteries exhibited vasospasm after SAH and became more severe on day 3 and 5. The elevated expression of TLR4 was detected after SAH and peaked on day 3 and 5. TLR4 is increasingly expressed in a parallel time course to the development of cerebral vasospasm in a rabbit experimental model of SAH.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2007.07.059