Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples

Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2007-10, Vol.71 (1), p.75-77
Main Authors: Riner, Diana K., Mullin, Andrew S., Lucas, Sasha Y., Cross, John H., Lindquist, H.D. Alan
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Centrifugation - methods
Cyclospora - isolation & purification
Cyclospora - physiology
Cyclospora cayetanensis
Cyclosporiasis - diagnosis
Detachment solution
Feces - parasitology
Flow cytometry
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Mycology
Oocysts
Renocal-sucrose gradient
Sucrose
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2007.06.021